Product Name :
|AccuEasy Flow Cytometry Kit||
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|Unit Price (1-4 Units)||Unit Price (5 units or more)||Inquire:|
|•||Price is for US enduser only. International price may vary. Please contact your local distributors for your price.|
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- The conventional flow cytometry method for analyzing cell surface markers on adherent cells requires detachment of cells from their culture surface before subjecting to antibody staining. Unfortunately, cell detachment introduces a significant stress to cells which can lead to changes in expression of cell surface markers. In addition, multiple centrifugation step often are required during the antibody staining, which is not only laborious, but also can lead to cell loss.
The AccuEasy flow cytometry kit offered by Biotium providing you a much simpler, more accurate and often more sensitive method for detecting cell surface markers on adherent cells than the conventional method. The AccuEasy method eliminates laborious centrifugation steps. More importantly, AccuEasy allows you to detect cell surface marker expression on cells in their native adherent state without perturbation caused by cell detachment. The AccuEasy method can yield substantially higher fluorescence signal for cell surface markers compared to conventional methods.
See our Flow Cytometry Brochure for more products for flow cytometry.
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HeLa cells grown in a 24-well plate were stained with CF488A-conjugated anti-transferrin receptor antibody (TfR) or CF488A-conjugated isotype control antibody (Iso) using either the AccuEasy protocol or a conventional staining protocol. In the AccuEasy protocol, cells were stained in the wells and detached for flow cytometry after staining using the AccuEasy cell lift solution. In the conventional protocol, cells were detached from the wells by scraping and stained in suspension. Mouse anti-TfR and isotype control antibodies (BD Biosciences) were conjugated to CF488A using Biotium's Mix-n-Stain antibody labeling kits. Fluorescence was analyzed using a BD FACSCalibur flow cytometer in the FL1 channel. Bars represent the signal to noise ratio (TfR mean fluorescence/Iso mean fluorescence).