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|Griess Reagent Kit||
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- Griess reagent is used to detect nitrite photometrically.
The reagent contains two chemicals, sulfanilic acid and
N-(1-naphthalenediamine). Under acidic conditions
sulfanilic acid is coverted by nitrite to a diazonium salt,
which readily couples with N-(1-naphthalenediamine)
to form a highly colored azo dye that can be detected at
548 nm (see below).
Under physiological conditions, NO is unstable and is rapidly oxidized to a mixture of nitrite and nitrate. In order to measure NO levels indirectly from nitrite, nitrate must be reduced to nitrite enzymatically using nitrate reductase (not included) before performing the assay so that the total amount of nitrite can be measured. The kit includes 50 mL ready-to-use Griess reagent, 1 mL nitrite standard solution (1 mM), and a detailed protocol. The volume of reagent provided is sufficient for 500 cuvette assays (3 mL assay volume), or 2500 96-well microplate assays (300 uL assay volume).
1) FASEB J. 7, 349 (1993); 2) Anal. Biochem. 126, 131(1982).
2. Smith, B. et al., Reduced in vitro immune response on titania nanotube arrays compared to titanium surface. Biomaterials Science(2012). DOI: 10.1039/c2bm00079b