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Peptidase substrates

Biotium offers many traditional fluorogenic peptidase substrates based on three fluorescent dyes: rhodamine 110 (R110), 7-amino-4-trifluoromethycoumarin (AFC) and 7-amino-4-mehylcoumarin (AMC or MCA). Before enzymatic hydrolysis, the substrates have no fluroescence or very weak fluorescence. Upon hydrolysis, the dye is released, increasing the fluorescence. In addition, Biotium offers a novel caspase-3 substrate, DEVD-NucView 488, which is suitable for detecting caspase-3 activity in live cells. The substrate enters into dying cells rapidly, where it is cleaved by caspases to release a DNA-binding dye, which stains the nucleus with green fluorescence. The unique features of NucView 488 substrate make it compatible with flow cytometry, microscopy and microplate reader analyses.

Cat#	Product Name	Unit Size	Unit Price
10202	Ac-DEVD-AMC (7-Amino-4-methylcoumarin, N-acetyl-L-aspartyl-Lglutamyl-L-valyl-l-aspartic acid amide)	5mg	$200
10206	Ac-DEVD-R110 (Rhodamine 110, bis-( N-acetyl-L-aspartyl-L-glutamyl-L-valyl-l-aspartic acid amide)	5mg	$231
10207	Ac-IETD-R110 (Rhodamine 110, bis-(N-acetyl-L-isoleucyl-L-glutamyl- L-threonyl-L-aspartic acid amide)	5mg	$231
10205	BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride)	5mg	$95
10208	BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride)	5mg	$158
10210	D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt)	2mg	$105
10225	R110-PEG (Rhodamine 110-PEG)	5 mg	$168
80103	Rhodamine 110 (high purity)	25 mg	$33
10211	Z-AAD-R110 (Rhodamine 110, bis-(N-CBZ-L-alanyl-L-alanyl-L-aspartic acid amide))	5 mg	$210
10220	Z-AA-R110-PEG (Rhodamine 110-PEG, N-CBZ-L-alanyl-L-alanine amide)	1 mg	$116
10201	Z-FR-AMC (7-Amino-4-methylcoumarin, N-CBZ-L-phenylalanyl-Larginine amide, hydrochloride)	25mg	$121
10209	Z-FR-R110 (Rhodamine 110, bis-(N-CBZ-L-phenylalanyl-L-arginine amide), dihydrochloride)	5mg	$95