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|GelRedTM Nucleic Acid Gel Stain, 10,000X in DMSO||
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- GelRedTM is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.
GelRedTM can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.
For more information, please see GelRed and GelGreen Frequently Asked Questions.
Biotium offers GelRedTM 10,000X solution in water (cat#41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRedTM 10,000X solution in DMSO for established users of GelRedTM in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRedTM 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).
- Safer than EB
- Easy disposal
- Extremely stable
- Simple to use
- Perfectly compatible with a standard UV transilluminator
- Perfectly compatible with downstream applications
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
Passed environmental safety tests for direct disposal down the drain or in regular trash
Much more sensitive than EtBr and SYBR Safe
Available in water, stable at room temperature for long-term storage and microwavable
Very simple procedures for either precast and post gel staining
GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
The Most Sensitive and Stable Precast Gel StainFigure 1.GelRedTM is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films. The Most Sensitive and Stable Post Gel Stain Figure 2. GelRedTM displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRedTMand SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer
Please also see our EvaGreenTM(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreenTM has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRedTM, EvaGreenTM has remarkable stability.
Note: *GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.
- Related Products:
1 kb DNA Ladder (100ng/uL) / 30 ug/300 ul (# 31021)
EvaGreen® dye, 20X in water / 5 x 1mL (# 31000)
GelRedTM Nucleic Acid Gel Stain, 10,000X in water / 0.5 mL (# 41003)
GelRedTM Nucleic Acid Gel Stain, 3X in water / 4.0 L (# 41001)
Ready-to-Use 1 kb DNA Ladder / 150 applications (1.5 ml) (# 31022)
TBE Buffer, 5X / 4 L (# 41006)
For Electrophoretic Mobility Shift Assay: 1. Liu,Y.,et al. Biochemistry, DOI: 10.1021/bi902050p, 2010, 2. Konate, K., et al. Biochemistry, DOI: 10.1021/bi901791x, 2010.1. Woźniakowski et al. Loop-mediated isothermal amplification for the detection of goose circovirus. Virology Journal 2012, 9:110