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CF™405S Dye

Outshining Alexa Fluor® 405!

  CF405S dye flyer
  CF dye selection guide

CF™405S is a blue fluorescent dye with an absorption peak wavelength that nearly coincides with the 405 nm blue diode laser line (Figure 1). In addition, the emission peak wavelength of the dye well centers within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF™405S dye results in much brighter signal than that using Alexa Fluor™ 405 (Figure 2).

A list of CF™405S-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 404/431 nm Extinction coefficient: 33,000 Molecular weight: 1,169 Flow cytometry laser line: 405 nm Microscopy laser line: 405 nm Direct replacement for: Alexa Fluor® 405, Cascade Blue® dye, DyLight™ 405 Advantages Much brighter than Alexa Fluor® 405 due to better compatibility with both excitation and emission windows on common instruments Highly water-soluble pH-insensitive
	Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD LSRII (405 laser, Em 450/50 nm). The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 1. Absorption and emission spectra of CF™405S and Alexa Fluor® 405 conjugated to goat anti-mouse IgG in PBS. The shaded area represents the wavelength window of the 450/50 filter on a BD LSR II flow cytometer.	Figure 3. HeLa cells stained with mouse-Golgin 97 and rabbit COX IV followed by goat anti-mouse CF405S (blue Golgi), goat anti-rabbit CF488A (green mitochondria) and CF555 phalloidin (red F-actin).

Table A. CF405S Dye-labeled Secondary Antibody Conjugates

Table B. Other CF405S dye-labeled Products

Table C. Reactive CF405S Dyes and Labeling Kits