CF™555 Dye
CF™555 is an orange-red fluorescent dye spectrally similar to Cy3 and Alexa Fluor®555. Like Alexa Fluor® 555, CF™555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF™555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining. Figures 1.x and 1.x compare CF™555, Cy®3 and Alexa Fluor® 555 in brightness and photostability. Available reactive forms of CF™555 dye include succinimidyl ester (SE), maleimide, methanethiosulfonate (MTS), amine and hydrazide. Biotium also provides CF™405M-labeled conjugates of various secondary IgG antibodies, streptavidin, phalloidin and a-bungarotoxin. See individual sections for details on these specific products. See Table A-C for the physical property of CF™ dyes.
Table A. CF555 Dye-labeled Secondary Antibody Conjugates
Cat# |
Product Name |
Unit Size |
Unit Price |
CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat) |
1 mg |
$185 |
|
CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL |
50 uL |
$75 |
|
CF555 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL |
50 uL |
$75 |
|
CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep) |
1 mg |
$185 |
|
CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL |
50 uL |
$75 |
|
CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep) |
1 mg |
$185 |
|
CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL |
50 uL |
$75 |
|
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL |
0.5 mL |
$180 |
|
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL |
50 uL |
$75 |
|
CF555 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL |
0.25 mL |
$120 |
|
CF555 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL |
50 uL |
$75 |
|
CF555 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL |
0.25 mL |
$120 |
|
CF555 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2mg/mL |
0.5 mL |
$180 |
|
CF555 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Guinea Pig IgG (H+L) |
1 mg |
$155 |
|
CF555 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL |
0.5 mL |
$150 |
|
CF555 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL |
50 uL |
$60 |
|
CF555 Goat Anti-Human IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Mouse IgG (H+L) |
1 mg |
$155 |
|
CF555 Goat Anti-Mouse IgG (H+L), 2 mg/mL |
0.5 mL |
$150 |
|
CF555 Goat Anti-Mouse IgG (H+L), 2 mg/mL |
50 uL |
$60 |
|
CF555 Goat Anti-Mouse IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Goat Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL |
0.5 mL |
$180 |
|
CF555 Goat Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
50 uL |
$85 |
|
CF555 Goat anti-mouse IgG1, (γ1), 2 mg/mL |
0.25 mL |
$195 |
|
CF555 Goat Anti-Mouse IgG2a (γ2a), 2mg/mL |
50 uL |
$85 |
|
CF555 Goat anti-mouse IgG2a, (γ2a), 2mg/mL |
0.25 mL |
$195 |
|
CF555 Goat Anti-Mouse IgG2b (γ2b), 2mg/mL |
50 uL |
$85 |
|
CF555 Goat anti-mouse IgG2b, (γ2b), 2mg/mL |
0.25 mL |
$195 |
|
CF555 Goat Anti-Rabbit IgG (H+L) |
1 mg |
$155 |
|
CF555 Goat Anti-Rabbit IgG (H+L), 2 mg/mL |
0.5 mL |
$150 |
|
CF555 Goat Anti-Rabbit IgG (H+L), 2 mg/mL |
50 uL |
$60 |
|
CF555 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL |
0.5 mL |
$180 |
|
CF555 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Rat IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL |
0.5 mL |
$180 |
|
CF555 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL |
50 uL |
$75 |
|
CF555 Goat Anti-Swine IgG (H+L) |
1 mg |
$155 |
|
CF555 Goat Anti-Swine IgG (H+L), 2 mg/mL |
0.5 mL |
$150 |
|
CF555 Goat Anti-Swine IgG (H+L), 2 mg/mL |
50 uL |
$60 |
|
CF555 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed |
1 mg |
$185 |
|
CF555 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL |
0.5 mL |
$180 |
|
CF555 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL |
50 uL |
$75 |
|
CF555 Rabbit Anti-Goat IgG (H+L) |
1 mg |
$155 |
|
CF555 Rabbit Anti-Goat IgG (H+L), 2 mg/mL |
0.5 mL |
$150 |
|
CF555 Rabbit Anti-Goat IgG (H+L), 2 mg/mL |
50 uL |
$60 |
Table B. Other CF555 dye-labeled Products
Cat# |
Product Name |
Unit Size |
Unit Price |
| Annexin V, CF555 conjugate, 50 ug/mL | 0.5 mL |
$220 |
|
| CF555 a-Bungarotoxin | 0.5 mg |
$315 |
|
| Phalloidin, CF555 conjugate | 300 U |
$305 |
|
| Phalloidin, CF555 conjugate | 50 U |
$65 |
Table C. Reactive CF555 Dyes and Labeling Kits
Cat# |
Product Name |
Unit Size |
Unit Price |
| CF555 SE protein labeling kit | 3 labelings |
$365 |
|
| CF555 succinimidyl ester | 1 umole |
$225 |
|
| Mix-n-Stain CF555 antibody labeling kit, 1x(20-50 ug) labeling | 1 labeling |
$105 |
|
| Mix-n-Stain CF555 antibody labeling kit, 1x(50-100 ug) labeling | 1 labeling |
$115 |
|
| Mix-n-Stain CF555 antibody labeling kit, 1X(5-20ug) labeling | 1 labeling |
$95 |
| **Price is for US enduser only. International price may vary. Please contact your local distributors for your price. |