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CF™594 Dye

Truly the brightest deep-red dye

  CF594 dye flyer
  CF dye selection guide

CF™594 is a deep red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye (Figure 1). On protein, CF™594 is significantly brighter than Alexa Fluor® 594 due to its high quantum yield and exceptional water solubility (Figure 2). CF™594 also has excellent photostability ideal for demanding applications, such as confocal and single molecular imagings (Figure 3). These properties make CF™594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful to combine with our blue fluorescent CF™350, green fluorescent CF™488A and far red CF™647 for multi-color imaging.
A list of CF™594-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 593/614 nm Extinction coefficient: 115,000 Molecular weight: ~730 Flow cytometry laser line: 532 or 594 nm Microscopy laser line: 594 nm Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight 594 and Cy™3. Advantages Yields the brightest antibody conjugates among spectrally similar dyes. Extremely photostable Highly water-soluble
	Figure 1. Absorption and emission spectra of CF™594 conjugated to goat anti-mouse IgG in PBS.
Figure 3. Jurkat cells were stained with intracellular CD3 followed by CF594 or AlexaFluor594 goat anti-mouse conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.
	Figure 2. Relative fluorescence of CF™594- and Alexa Fluor® 594-goat anti-mouse conjugates as a function of number of dye per protein or degree of labeling (DOL). Fluorescence was measured at each dye’s emission maximum in PBS using 594 nm excitation.

Table A. CF594 Dye-labeled Secondary Antibody Conjugates

Table B. Other CF594 dye-labeled Products

Table C. Reactive CF594 Dyes and Labeling Kits