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Fluorescent CF® Dyes CF®647 & CF®647Plus Dyes

CF®647 & CF®647Plus

Highly fluorescent far-red dyes
  • Brighter than Cy®5
  • Water soluble & pH-insensitive
  • CF®647 validated in multi-color STORM
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 & CF®647Plus
Highly fluorescent far-red dyes
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with superior chemical stability for TSA and photostability for microscopy
RPE-Astral™775
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®647 is a cyanine-based far-red fluorescent dye spectrally similar to Cy®5 and Alexa Fluor® 647, with excellent brightness. CF®647 has been validated in multi-color super-resolution imaging by STORM (learn more about CF® Dyes for super-resolution imaging).

CF®647Plus was designed as an improved and brighter alternative to Biotium's original CF®647 Dye. CF®647Plus is spectrally identical to CF@647 and is available with select Biotium Choice antibodies that have been optimized and validated in-house for flow cytometry. 

For far-red dyes with excellent brightness and photostability for microscopy applications other than STORM, see CF®633 or CF®640R.

See products available with CF®647. Additional reactive dyes or conjugates may be added on request, contact [email protected] to inquire.

CF®647 Technical Summary

  • Abs/Em Maxima: 652/668 nm
  • Extinction coefficient: 240,000
  • Molecular weight: ~1058
  • Laser line: 633 or 635 nm
  • Alternative for: Cy®5, Alexa Fluor® 647, DyLight® 649
  • Fluorescence lifetime:
    τ (ns)/free acid in PBS pH 7.4, ε (ns) = 1.07 ± 0.05
    τ (ns) /S.Ab§ = 1.195

Visit our FAQ for more fluorescence life time information on CF® Dyes.

CF®647Plus Technical Summary

  • Abs/Em Maxima: 652/668 nm
  • Extinction coefficient: 240,000
  • Molecular weight: ~1426
  • Laser line: 633 or 635 nm
  • Alternative for: Cy®5, Alexa Fluor® 647, DyLight® 649

Browse Biotium Choice antibodies available conjugated to CF®647Plus

Figure 1. Normalized absorption and emission spectra of CF®647 goat anti-mouse conjugate in PBS.
Figure 2. Apoptotic Jurkat cell stained with NucView® 488 (green) and CF®647 Annexin V (magenta).
Figure 3. Comparison of microtubule imaging using conventional wide-field microscopy with STORM using CF®647 dye conjugate. Images courtesy of Sam Kenny and Pro... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®640R Dye
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CF®660C & CF®660R

CF is a registered trademark of Biotium. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

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