FURA-2 and Furaptra
Fura-2 is a widely used UV-excitable fluorescent calcium indicator.1-5 Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. The indicator is typically excited at 340 nm and 380 nm respectively and the ratio of the fluorescent intensities corresponding to the two excitations is used in calculating the intracellular concentrations. Measurement of calcium concentration using this ratioing method avoids interference due to uneven dye distribution and photobleaching (Bright, G.R., et al, in Fluorescence Microscopy of Living Cells in Culture, Part B, (Methods in Cell Biology, Vol. 30), Academic Press (1989) p. 157). Fura-2 has been used in many cellular systems and applications, particularly in microscopic imaging.
Furaptra, which is also commonly called Mag-Fura-2, was originally developed as a fluorescent magnesium indicator. However, it is an excellent indicator for high calcium in the range of 1µM to 100 µM.6,7 Fura-2 and Furaptra share the same chromophore and as a result have similar spectral change in response to calcium concentration change.
Cat# |
Product Name |
Unit Size |
Unit Price |
59100 |
kit |
$130 |
|
90082 |
10mL |
$18 |
|
59002 |
100mg |
$66 |
|
50029 |
1ml |
$138 |
|
50030 |
1mg |
$105 |
|
50031 |
1mg |
$105 |
|
50032 |
1mg |
$105 |
|
50033 |
10x100ug |
$140 |
|
50033-1 |
20x50ug |
$150 |
|
50034 |
1mg |
$115 |
|
50035 |
1mg |
$130 |
|
50036 |
1mg |
$130 |
|
50037 |
10x100ug |
$185 |
|
50038 |
1mg |
$170 |
|
50039 |
20x50ug |
$205 |
|
50045 |
1mg |
$135 |
|
59003 |
100mg |
$95 |
|
59004 |
1ml |
$12 |
|
59005 |
30mL |
$43 |
|
59007 |
1 mg |
$132 |
|
59000 |
1g |
$17 |