Product types > Fluorescent Calcium Indicators and Related Products > Indo-1

Indo-1

Indo-1 is a UV-excitable fluorescent Ca²⁺ indicator that can be optimally excited by the 351-356 nm spectral lines of the argon-ion laser. The emission maximum of the indicator shifts from ~475 nm in Ca²⁺-free medium to ~400 nm in Ca²⁺-saturated medium (Figure 1.9). Ca²⁺ concentration can thus be determined by ratioing the fluorescence intensities at the two wavelengths. As with Fura-2, this ratioing technique avoids problems associated with uneven dye distribution, cell or tissue thickness and photobleaching. Indo-1 has been widely used in flow cytometry studies.

Cat#	Product Name	Unit Size	Unit Price
59100	Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)	kit	$130
90082	DMSO, anhydrous (Dimethyl sulfoxide, or methyl sulfoxide)	10mL	$18
59002	EDC (EDAC; 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride))	100mg	$66
50040	Indo-1, pentaammonium salt	1mg	$100
50041	Indo-1, pentapotassium salt	1mg	$100
50042	Indo-1, pentasodium salt	1mg	$100
50043	Indo-1, AM ester	10x100ug	$140
50043-1	Indo-1, AM ester	20x50ug	$150
50044	Indo-1, AM ester	1mg	$115
59003	TPEN (Full name: tetrakis-(2-pyridylmethyl)ethylenediamine)	100mg	$95
59004	Pluronic F-127 20% solution in DMSO	1ml	$12
59005	Pluronic F-127, 10% in sterile H2O	30mL	$43
59007	Ionomycin,calcium salt	1 mg	$132
59000	Pluronic F-127	1g	$17