800.304.5357

Product Types > Natural Products and Their Fluorescent Conjugates > Natural Products and Their Fluorescent Conjugates > Phalloidin, Sulforhodamine 101 (Texas-Red) conjugate



Product Name :   
     Phalloidin, Sulforhodamine 101 (Texas-Red) conjugate
MSDS
Catalog Number :   
     00033
Unit Size :   
     300U    
Availability :   
     In Stock

Pricing Information:

Unit Price (1-4 Units) Unit Price (5 units or more) Inquire:
$230 $207 quote@biotium.com
 
  • Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

  • Description
  • Related Products
  • References
  • Phalloidin and phallacidin are collectively called phallotoxins, which are bicyclic peptides isolated from the deadly Amanita phalloides mushroom. Phallotoxins bind specifically to F-actin filaments with high affinity(1). Fluorescently labeled phallotoxins are very useful tool for investigating the distribution of F-actin. Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants and animals. Phallotoxins shift the F-actin monomer/polymer equilibrium toward the polymer, lowering the critical concentration for polymerization up to 30-fold(3,4). Phallotoxins also stabilize F-actin filaments, inhibiting depolymerization by cytochalasins, potassium iodide and elevated temperatures. Moreover, phallotoxin-labeled actin filaments remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin substrates(5,6). Fluorescent phallotoxins can also be used to quantify the amount of F-actin in cells(7,8).

    Sulforhodamine 101 (Texas-Red) Phalloidin binds to F-actin with nanomolar affinity. The red fluorescent phalloidin is typically applied to fixed and permeabilized cells but can also be loaded into live cells via cationic liposomes.

    • lex\lem(MeOH) = 591/608nm
    • Red solid soluble in MeOH
    • Store at -20°C and protect from light


    Chemical Structure:
  • Reference:
    1. Wieland, T. in Phallotoxins, Springer-Verlag, New York (1986); 2. J Muscle Res Cell Motil 9, 370 (1988); 3. Methods Enzymol 85, 514 (1982); 4. Eur J Biochem 165, 125 (1987); 5. Nature 326, 805 (1987); 6. Proc Natl Acad Sci USA 83, 6272 (1986); 7. Blood