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Product Types > Nucleic Acid Stains and Products for Genomics Studies > Nucleic Acid Stains and Products for Genomics Studies > EvaGreen® dye 25 mM in DMSO (available to licensees only)



Product Name :   
     EvaGreen® dye 25 mM in DMSO (available to licensees only)
Safety Report
MSDS
Catalog Number :   
     31002
Unit Size :   
     1 x 1mL    
Availability :   
     In Stock

Pricing Information:

Unit Price (1-4 Units) Unit Price (5 units or more) Inquire:
$420 $378 quote@biotium.com
 
  • Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

  • Description
  • References
  • Evagreen® Dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), routine solution DNA quantification and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® dye Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).

    The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) application. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in more robust signal for both PCR and melt curve analysis.

    Features:

    • Low PCR inhibition

      • Exhibit much less PCR inhibition than SYBR Green I via a smart "release-on-demand" DNA-binding technology

    • Highly Sensitive

      • Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals

    • Nonmutagenic and noncytotoxic

      • Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes

    • Compatible with Fast PCR protocol

      • Minimal interference to PCR makes it possible to significantly shorten the chain extension time

    • Compatible with multiplex PCR

      • No dye migration from amplicon to amplicon when used at the recommended concentration

    • Unsurpassed Thermal Stability, Hydrolytical Stability and Photostability

      • No detectable dye decomposition in PCR buffer at 95-100?C for 48 hours; highly stable under either alkaline or acidic condition; withstand repeated freeze-thaw cycles

    • Spectrally similar to SYBR Green I

      • Compatible with all major brand qPCR instruments and enzyme systems