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Product Types > Nucleic Acid Stains and Products for Genomics Studies > Nucleic Acid Stains and Products for Genomics Studies > AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards



Product Name :   
     AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards
MSDS
Catalog Number :   
     31006
Unit Size :   
     1000 assays    
Availability :   
     In Stock

Pricing Information:

Unit Price (1-4 Units) Unit Price (5 units or more) Inquire:
$225 $202.5 quote@biotium.com
 
  • Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

  • Description
  • Related Products
  • References
  • The AccuBlue (formerly MagicBlue) High Sensitivity dsDNA Quantitation Kit offers ultra-sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a linear detection range from 0.2 - 100 ng with sufficient reagents to perform 1000 assays based on a 96-well microplate format. The kit includes AccuBlue High Sensitivity Quantitation Solution components and a set of 8 pre-diluted calf thymus dsDNA standards. AccuBlue High Sensitivity Quantitation Solution also is available separately (Catalog #31008) for customers who wish to use their own DNA standards.

    The AccuBlue High Sensitivity assay is superior to PicoGreen or Quant-iT dsDNA detection kits by having a similar detection range but with more convenience and better safety. The high sensitivity reagent is impermeable to cell membranes, whereas PicoGreen or the high sensitivity Quant-iT DNA-binding dye readily crosses cell membranes. Moreover, the reagent is extremely stable. AccuBlue can be conveniently stored at 4C, unlike PicoGreen or Quant-iT reagents, which must be aliquotted for storage to avoid multiple freeze-thaw cycles.

    Biotium's AccuLite 470 Mini Fluorometer includes a program for use with the AccuBlue High Sensitivity assay. The assay (Ex/Em 485/530 nm) also is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers such as the Qubit from Invitrogen.

    Please also see our AccuBlue Broad Range dsDNA Quantitation Kit for quantitation of 2-2000 ng and our AccuClear Ultra High Sensitivity dsDNA Quantitation Kit for quantitation of 0.03-250 ng DNA (related products).

    AccuBlue kits are intended for quantitation of purified dsDNA samples. For cell quantitation assays, please see our Apoptosis, Necrosis and Cell Viability Assays Brochure.

    AccuBlue technology is covered by U.S. Patent No. 8,148,515.




  • Reference:
    1. Burrell, A., et al. Discovery of nuclear and plastid microsatellites, and other key genomic information, in the rare endemic plant (Caulanthus amplexicaulis var. barbarae) using minimal 454 pyrosequencing. Conservation Genet Resour DOI 10.1007/s12686-011-9450-3.

    2. Bravo, N., et al., Real-Time PCR in faecal samples of Triatoma infestans obtained by xenodiagnosis: proposal for an exogenous internal control, Parasites & Vectors 2012, 5:59 doi:10.1186/1756-3305-5-59

    3. R. Nian, et al., Void Exclusion of Antibodies by Grafted- Ligand Porous Particle Anion Exchangers, Journal of Chromatography A (2010), doi:10.1016/j.chroma.2013.01.065

    4. Klagges C. et al., Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars. PLoS ONE 8(1): e54743. doi:10.1371/journal.pone.0054743

    5. H.T. Gan, et al., Characterization and Removal of Aggregates Formed by Nonspecific Interaction of IgM Monoclonal Antibodies with Chromatin Catabolites During Cell Culture Production, Journal of Chromatography A (2013), http://dx.doi.org/10.1016/j.chroma.2013.03.028

    6. Waggoner JJ, Abeynayake J, Sahoo MK, Gresh L, Tellez Y, et al. (2013) Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses. PLoS Negl Trop Dis 7(4): e2116. doi:10.1371/journal.pntd.0002116