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Product Types > > EvaGreen® dye, 20X in water



Product Name :   
     EvaGreen® dye, 20X in water
Safety Report
MSDS
Catalog Number :   
     31000
Unit Size :   
     5 x 1mL    
Availability :   
     In Stock

Pricing Information:

Unit Price (1-4 Units) Unit Price (5 units or more) Inquire:
$175 $157.5 quote@biotium.com
 
  • Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

  • Description
  • Related Products
  • References
  • EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), routine solution DNA quantification and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).

    The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) application. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in more robust signal for both PCR and melt curve analysis.

    Features:

    • Low PCR inhibition

      • Exhibit much less PCR inhibition than SYBR Green I via a smart "release-on-demand" DNA-binding technology

    • Highly Sensitive

      • Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals

    • Nonmutagenic and noncytotoxic

      • Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes

    • Compatible with Fast PCR protocol

      • Minimal interference to PCR makes it possible to significantly shorten the chain extension time

    • Compatible with multiplex PCR

      • No dye migration from amplicon to amplicon when used at the recommended concentration

    • Unsurpassed Thermal Stability, Hydrolytical Stability and Photostability

      • No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstand repeated freeze-thaw cycles

    • Spectrally similar to SYBR Green I

      • Compatible with all major brand qPCR instruments and enzyme systems

    For related products, download our Genomics and Proteomics Products Brochure.




  • Reference:
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    2. Luchi, N., et al. High-Resolution Melting Analysis: a new molecular approach for the early detection of Diplodia pinea in Austrian pine. Fungal Biology doi:10.1016/j.funbio.2011.05.005 (2011).

    3. Mao, et al. Characterization of EvaGreen Dye and the implication of its physicochemical properties for qPCR applications. BMC Biotechnology 7, 76 (2007).

    4. Eischeid A.C. SYTO dyes and EvaGreen outperfrom SYBR Green in real-time PCR. BMC Research Notes doi. 10.1186/1756-0500-4-263 (2011).

    5. Kozina, V., et al. A One-Step Real-Time Multiplex PCR for Screening Y-Chromosomal Microdeletions without Downstream Amplicon Size Analysis. PLoS ONE6(8): e23174. doi:10.1371/journal.pone.0023174

    6. Jin, D. et al., Rapid molecular identification of Listeria species by use of real-time PCR and high-resolution melting analysis. FEMS Microbiology Letter Volume 330, Issue 1, pages 72-80. Doi: 10.1111/j.1574-6968.2012.02535.x(2012)

    7. Pascale May-Panloup, et al. Molecular characterization of corona radiata cells from patients with diminished ovarian reserve using microarray and microfluidic-based gene expression profiling. Human Reproduction, Vol.0, No.0 pp. 1–15, 2012 doi:10.1093/humrep/der431

    8. S. J., et al., Detection of eight common β-globin gene mutation in thalassemia major patients using real time polymerase chain reaction (PCR)-high resolution melting and EvaGreen™ dye. African Journal of Biotechnology Vol. 11(2), pp. 448-459, 5 January, 2012 DOI: 10.5897/AJB10.1167

    9.Reina-Pinto, J. et al., Misexpression of FATTY ACID ELONGATION1 in the Arabidopsis Epidermis Induces Cell Death and Suggests a Critical Role for Phospholipase A2 in This Process. The Plant Cell, Vol. 21, : 1 252-1 272, April 2009

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