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Do CellBrite™ dyes specifically stain the plasma membrane?

Original CellBrite™ Cytoplasmic Membrane Stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain fixed cells or artificial lipid bilayers.

CellBrite™ Fix Membrane Stains are fluorogenic membrane dyes that react covalently with proteins on the cell surface. They have better water solubility than original CellBrite™ dyes and as a result generally give more even staining of the cell surface compared to original CellBrite™ dyes. CellBrite™ Fix are well retained after fixation and permeabiliation or methanol fixation. However, CellBrite™ Fix dyes cannot be used to stain the plasma membrane of cells after they are already fixed (the dyes stain intracellular membranes of fixed cells).

For all membrane stains, if cells are returned to culture after staining, membrane internalization will occur over time, resulting in predominantly intracellular staining.

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

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