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What’s the difference between CellBrite™, CellBrite™ NIR, and CellBrite™ Fix?

CellBrite™ and CellBrite™ Fix dyes are designed to label cell membranes. Original CellBrite™ dyes contain lipophilic carbocyanine dyes (DiB, Neuro-DiO, DiI, or DiD). These dyes accumulate in membrane lipids where they become fluorescent. Like other membrane dyes like VyBrant® DiO, VyBrant® DiI, ViBrant® DiR, CellMask™, CellVue® Claret, or PKH dyes, original CellBrite™ dyes can be fixed with formaldehyde, but are poorly retained after permeabilization with detergents or fixation with alcohol. CellBrite™ NIR dyes are CellBrite™ dyes with near-infrared fluorescence compatible with small animal NIR imaging systems.

CellBrite™ Fix dyes are a novel class of dye for labeling the cell surface. They are fluorogenic dyes that rapidly accumulate in the plasma membrane, where they react covalently with the cell surface. As a result, surface staining is well-retained after permeabilization or methanol fixation, with only a slight increase in intracellular fluorescence compared to formaldehyde fixation alone. CellBrite™ Fix dyes have better water solubility than classic lipophilic dyes, and as a result they yield much more uniform staining compared to lipophilic carbocyanine dyes like DiO and DiI. CellBrite™ Fix dyes are non-toxic and do not readily transfer between cells. They also can be used to stain yeast and bacteria (gram-positive or gram-negative).

Vybrant® and CellMask™ are trademarks of Thermo Fisher Scientific. CellVue® is a registered trademark of Millipore Sigma.

Category: CellBrite™ Cytoplasmic Membrane Stains

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