FAQs

FAQs

What is the ROX concentration in Fast Probe Master Mix?

The ROX concentration is 35 nM which is suitable for low ROX instruments. If you are using a...

FAQs

What is the difference between EvaGreen® Dye and EvaGreen® Plus Dye?

Our original EvaGreen® Dye is a saturating dsDNA binding dye that is superior for quantitative real-time PCR (qPCR),...

FAQs

What Concentration of EvaGreen should be used for LAMP?

0.5X to 1X EvaGreen® is commonly used for LAMP applications when fluorescent signal is read by instrumentation. For...

FAQs

What are the concentrations of EvaGreen® Dye solutions?

EvaGreen® Dye is supplied at 20X (25 uM) in water (cat. no. 31000) or 2000X (2.5 mM) in...

FAQs

Can EvaGreen® Dye, 2000X in DMSO be diluted in water?

If necessary, EvaGreen® Dye, 2000X in DMSO can be diluted in water or master mix for qPCR for...

FAQs

Which EvaGreen® product, 20X in water or 2000X in DMSO, is best for qPCR?

For most qPCR applications, we recommend using EvaGreen® Dye, 20X in water (31000), or one of our 2X...