FAQs

FAQs

What is the mechanism of RedDot™ 1 or RedDot™ 2 binding to DNA? Are the dyes intercalators, or minor groove binders?

The mechanism of binding for RedDot™ 1 and RedDot™ 2 to DNA has not been characterized. However, based...

FAQs

What is the mechanism of staining for potential-independent mitochondrial dyes?

Mitochondrial dyes, including MitoView™ Mitochondrial Dyes, are positively charged and lipophilic. They passively diffuse a...

FAQs

What mounting media can be used with LipidSpot™ Lipid Droplet Stains?

Mounting medium can alter the staining of lipophilic dyes like LipidSpot™, due to interaction of the dyes with...

FAQs

Are TrueBlack® or TrueBlack® Plus lipofuscin autofluorescence quenchers compatible with lipid droplet stains like BODIPY or LipidSpot®?

TrueBlack® and TrueBlack® Plus are hydrophobic in nature and quench lipofuscin autofluorescence mainly through hydrophobic interac...

FAQs

What is the staining mechanism of MitoView™ and other mitochondrial dyes? Where do they localize in the organelle?

Cationic dyes are widely used as mitochondrial probes. They accumulate within the cell and preferentially localize in the...

FAQs

Can cells be fixed after staining with MitoView™ dyes? Can MitoView™ dyes be used to stain cells or tissue sections that are already fixed?

Most of our MitoView™ dyes are sensitive to mitochondrial membrane potential to some degree, and therefore are recommended....