FAQs

FAQs

There is SDS in the assay buffer of the AccuOrange™ protein concentration kit, but it says the assay can’t tolerate SDS. Why?

Even though AccuOrange™ buffer does contain SDS, which is required for the dye to bind proteins, the assay...

FAQs

Can I use One-Step Stains to stain radiolabeled proteins on an SDS-PAGE gel, followed by drying, and autoradiography?

Gels stained with One-Step Blue® can be dried just like gels stained with Coomassie. The stain will not...

FAQs

What is the mechanism of the AccuOrange™ Protein Quantitation kit?

The AccuOrange™ assay is a fluorescent dye-based assay. The dye binds to proteins primarily through hydrophobic interaction...

FAQs

Is protein staining on SDS-PAGE gels with the One-Step protein stains compatible with western blotting?

We do not recommend staining proteins on the polyacrylamide gel with the One-Step protein gel stains before western...

FAQs

Can the Lumitein™ Protein Gel Stain be used for staining native PAGE gels?

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be...

FAQs

Why are my protein marker bands not visible with the Lumitein™, One-step Lumitein™, or One-step Lumitein™ UV gel stains?

Use of pre-stained protein markers can quench fluorescent protein stains. Switching to an unstained marker should resolve the...