FAQs

FAQs

Do secondary antibodies react against other isotypes?

Unless specified as isotype-specific, anti-IgG secondary antibodies are raised against whole immunoglobulin, so they are expected ...

FAQs

Is it vital to use serum from the same species as the secondary antibody for blocking?

No, you don't always need to use serum from the same species as the secondary antibody for blocking....

FAQs

What are F(ab) fragment-specific secondary antibodies, and when would they be used?

F(ab) fragment-specific antibodies react with the light chains and not the heavy chains of immunoglobulins from the target...

FAQs

What is the difference between F(ab) and F(ab’)2, and when would they be used?

F(ab’)2 is a fragment of IgG that is prepared by pepsin digestion of IgG. F(ab’)2 fragment is the...

FAQs

Do I have to use a secondary antibody that is specific to the isotype of my primary antibody? For example, if my primary is IgG2a isotype, do I have to use anti-IgG2a to detect it?

No, antibodies designated as anti-IgG (H+L) with no subtype specified will cross react with all sub-types of IgG,...

FAQs

Do I have to use secondary antibodies raised in the same host species?

As a rule, we recommend using secondary antibodies from the same host for multiplex staining (for example, combining...