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Biotin-XX-α-Bungarotoxin

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

Product Attributes

Toxin

Alpha-bungarotoxin

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Tissue staining

Cell permeability

Membrane impermeant

Detection method/readout

Microscopy

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Conjugation

Biotin

Storage Conditions

Store at -10 to -35 °C, Protect from light

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

A potent neurotoxin that is an inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). It binds at the neuromuscular junction with high affinity and is often used to study neuromuscular junctions by blocking cholinergic receptors. The toxin also blocks α7 receptors, located in the central and peripheral nervous systems. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

  • A polypeptide snake toxin
  • Blocks and inhibits cholinergic receptors
  • Biotinylated for avidin or streptavidin reactivity and signal amplification
  • White solid soluble in water

Biotinylated α-bungarotoxin is useful in the affinity column isolation of the nicotinic AChR using an avidin or streptavidin agarose (1). Nicotinic AChR labeled with biotin-XX-α-bungarotoxin can be localized using enzyme or fluorophore-labeled conjugates of avidin or streptavidin (2,3).

We also offer unlabeled α-bungarotoxin and CF® Dye α-Bungarotoxin conjugated to our next-generation fluorescent dyes. See our complete selection of α-Bungarotoxin Products below.

α-Bungarotoxin & Conjugates

α-Bungarotoxin, CF® Dye and Other Conjugates

ConjugationEx/EmSizeCatalog No.Dye Features
UnconjugatedN/A1 mg00010-1
Biotin-XXN/A0.5 mg00017
CF®405S404/431 nm100 ug00002-100ugCF®405S Features
0.5 mg00002
CF®488A490/515 nm100 ug00005-100ugCF®488A Features
0.5 mg00005
CF®543 541/560 nm100 ug00026-100ugCF®543 Features
0.5 mg00026
CF®555555/565 nm100 ug00018-100ugCF®555 Features
0.5 mg00018
CF®568562/583 nm100 ug00006-100ugCF®568 Features
0.5 mg00006
CF®594596/614 nm100 ug00007-100ugCF®594 Features
0.5 mg00007
CF®633630/650 nm100 ug00009-100ugCF®633 Features
0.5 mg00009
CF®640R642/662 nm100 ug00004-100ugCF®640R Features
0.5 mg00004
CF®680R680/701 nm100 ug00003-100ugCF®680R Features
0.5 mg00003
Fluorescein
(FITC)
494/518 nm0.5 mg00011
10 x 50 ug00013
Tetramethylrhodamine
(TRITC)
553/577 nm0.5 mg00012
10 x 50 ug00014
Sulforhodamine-101
(Texas Red®)
593/613 nm0.5 mg00015
10 x 50 ug00016
CF is a registered trademark of Biotium, Inc. Texas Red is a registered trademark of Thermo Fisher Scientific.

References

  1. PLoS One, 8, 7, (2013), DOI: 10.1371/journal.pone.0070338
  2. Am J of Pathol, 177, 2509 (2010), DOI: 10.2353/ajpath.2010.100243
  3. Cell Tissue Biol, 4, 258, (2010), DOI: 10.1134/S1990519X10030077
  4. Neuroscience, 174, 234, (2009), DOI: 10.1016/j.neuroscience.2010.11.016
  5. J Cell Biol, 150, 1385, (2000), DOI: 10.1083/jcb.150.6.1385
  6. Neuron, 23, 675, (1999), DOI: 10.1016/s0896-6273(01)80027-1
  7. Neuron, 12, 167, (1994), DOI: 10.1016/0896-6273(94)90161-9
  8. J Cell Biol, 125, 661, (1994), DOI: 10.1083/jcb.125.3.661
  9. J Biol Chem, 268, 25108, (1993), PMID: 8227074
  10. Muscle Nerve, 5, 140, (1982), DOI: 10.1002/mus.880050211
  11. PNAS 77, 4823, (1980), DOI: 10.1073/pnas.77.8.4823
  12. Science, 196, 540, (1977), DOI: 10.1126/science.850796
 

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