CF514 Tyramide

CF514 Spectra
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Product Description

This  CF™514  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal.  CF™514 can be detected using a standard fluorescein filter set. By using instruments that collect spectral distribution information, the signal from CF™514 can be distinguished from CF™488A dye using linear unmixing software.


Additional Information


0.5 mg

MSDS (PDF): MSDS CFdye and Biotin Tyramide

Protocol (PDF): PI-CF Dye or BiotinTyramide

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life