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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

Highly cross-adsorbed goat anti-mouse IgG (H+L) secondary antibody with single CF® Dye label for STORM super-resolution microscopy.

Product Attributes

Antibody reactivity (target)

Mouse IgG

Clonality

Polyclonal

Host species

Goat

Cross adsorption

Bovine, Horse, Human, Rabbit, Swine

Antibody type

Secondary

Concentration

1 mg/mL

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide

Species reactivity

Mouse

Secondary/tag antibody applications

IF (cells or tissue sections), STORM

Conjugation
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500 uL
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Product Description

This is a highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody that has a low degree of labeling (DOL) with one of our bright and photostable CF® Dyes.

  • CF® Dye single label secondary antibody ideal for STORM imaging
  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 9 bright and photostable CF® Dyes

Secondary antibodies with a low DOL, or number of dye molecules per antibody molecule, have been reported to be optimal for STORM (Bittel et al. (2015) Proc. SPIE 9331). This product is prepared by single labeling (DOL=1) of highly cross-adsorbed goat anti-mouse IgG (H L) with a selection of compatible CF® Dyes for (d)-STORM super-resolution microscopy. To minimize cross-reactivity, the antibody has been adsorbed against human, bovine, horse, rabbit, and swine serum.

Learn more about CF® Dyes for super-resolution microscopy.

Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

ConjugationEx/EmSizeCatalog No.Dye Features
CF®505505/519 nm50 uL (50 ug)20876-50uL
0.5 mL (500 ug)20876-500uL
CF®535ST535/568 nm50 uL (50 ug)20821-50uLCF®535ST Features
0.5 mL (500 ug)20821-500uL
CF®568562/583 nm50 uL (50 ug)20800-50uLCF®568 Features
0.5 mL (500 ug)20800-500uL
CF®583R586/609 nm50 uL (50 ug)20792-50uLCF®583R Features
0.5 mL (500 ug)20792-500uL
CF®597R597/619 nm50 uL (50 ug)20796-50uLCF®597R Features
0.5 mL (500 ug)20796-500uL
CF®647650/665 nm50 uL (50 ug)20808-50uLCF®647 Features
0.5 mL (500 ug)20808-500uL
CF®660C667/685 nm50 uL (50 ug)20812-50uLCF®660C Features
0.5 mL (500 ug)20812-500uL
CF®680681/698 nm50 uL (50 ug)20817-50uLCF®680 Features
0.5 mL (500 ug)20817-500uL
CF®750755/777 nm50 uL (50 ug)20825-50uLCF®750 Features

CF® Dye Secondary Antibodies, Single Label for STORM

DyeDonkey Anti-GoatDonkey Anti-Guinea PigDonkey Anti-MouseDonkey Anti-RabbitGoat Anti-MouseGoat Anti-Rabbit
Cross-adsorptionCk, GP, Hs, Hu, Ms, Rb, Rt, SHmBv, Ck, Gt, Hs, Hu, Ms, Rb, Rt, Shp, SHmBv, Ck, Gt, GP, Hs, Hu, Rb, Rt, Shp, SHmBv, Ck, Gt, GP, Hs, Hu, Ms, Rt, Shp, SHmBv, Hs, Hu, Rb, SwHu, Ms, Rt
CF®505208802088120878208792087620877
CF®535ST20823208242082120822
CF®568208362083820802208032080020801
CF®583R20794207952079220793
CF®597R20798207992079620797
CF®647208292083720810208112080820809
CF®660C20815208162081220813
CF®68020819208202081720818
CF®75020827208282082520826
Bv: bovine; Ch: chicken; Gt: goat; GP: guinea pig; Hs: horse; Hu: human; Ms: mouse; Rb: rabbit; Sh: sheep; SHm: Syrian hamster; Sw: swine; Rt: rat

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® Dye references.

  1. Proc. SPIE 9331, (2015), DOI: 10.1117/12.2083209
 

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