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RFP Polyclonal Rabbit Antibody

Polyclonal rabbit anti-RFP antibody labeled with our superior CF® dyes.

Product Attributes

Clonality

Polyclonal

Antibody type

Tag Antibody

Concentration

1 mg/mL

Host species

Rabbit

Antibody reactivity (target)

RFP

Cross adsorption

Human, Mouse, Rat

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Size
Catalog #
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Qty
100 uL
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Product Description

This is a polyclonal rabbit anti-RFP antibody labeled with our superior CF® dyes. The antibody is designed to detect red fluorescent protein (RFP) and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. To minimize cross reactivity, the antibodies are cross-adsorbed against human, mouse, and rat proteins.

  • Cross-adsorbed for specific staining with minimal background
  • Available in 7 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology

See our full selection of anti-tag and anti-hapten antibody conjugates.

RFP Polyclonal Rabbit Antibody

ConjugationEx/EmSizeCatalog No.Dye Features
CF®488A490/515 nm100 uL (100 ug)20421CF®488A Features
CF®543541/560 nm100 uL (100 ug)20476CF®543 Features
CF®568562/583 nm100 uL (100 ug)20477CF®568 Features
CF®594593/614 nm100 uL (100 ug)20422CF®594 Features
CF®640R642/662 nm100 uL (100 ug)20423CF®640R Features
CF®660R663/682 nm100 uL (100 ug)20478CF®660R Features
CF®680R680/701 nm100 uL (100 ug)20479CF®680R Features

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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