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Coelenterazine (native)

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase.

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CAS number

55779-48-1

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Product Description

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM (References 1-4). Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) (5) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues (6-9). Biotium also offers water soluble Aquaphile™ Coelenterazine. See the coelenterazine flyer under downloads for a comparison of coelenterazine analogs.

  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C26H21N3O3
  • MW: 423
  • [55779-48-1]

References

For in-vivo imaging:

For reporter assay:

1. Diabetologia (2011) doi: 10.1007/s00125-011-2133-5 (2011).

2. PLoS ONE 6(2), e16873 (2011).
3. Chemistry and Biology 17, 1018 (2010).
4. Oncogene. 30(1), 77 (2011).
5. Cell Mol Neurobiol (2010) doi: 10.1007/s10571-010-9572-2
6. Molecular Imaging and Biology (2010) doi: 10.1007/s11307-010-0325-x
7. Molecular Cancer (2009) doi:10.1186/1476-4598-8-45
8. Analytical Biochemistry (2007) doi: 10.1016/j.ab.2007.08.038
9. The Plant Journal Vol. 48, 138-52 (2006).
10. Clinical Cancer Research 11, 4487-94 (2005).
11. Journal of Virology 79(14), 8909-19 (2005).
12. Cancer Research 64, 2113-9 (2004).
13. Cancer Research 64, 1323-30 (2004).
14. PNAS (2004) doi:10.1073/pnas.0304326101
15. Molecular Imaging 3 (1), 43-54 (2004).
16. Cancer Research 64, 6684-92 (2004).
17. Oncogene 22, 6865-72 (2003).
18. PNAS (2003) doi:10.1073/pnas.2435285100
19. Cancer Research 63, 1160-5 (2003).
20. Luminescence 2002 17(2), 77-115 (2002).
21. PNAS (2001) doi:10.1073/pnas.012611099
22. Protoplasma 218, 1-23 (2001).
23. Free Radic. Biol. Med. 28, 1232 (2000).
24. PNAS 96, 151 (1999).
25. Circ. Res. 84, 1203 (1999).
26. Meth. Cell Biol. 40, 305 (1994).
27. Cell Calcium, 14, 373 (1993).
28. J Cell Biol 115, 1259(1991).
29. Meth. Enzymol. 172, 164 (1989).

 

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