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DAPI in H2O, 10 mg/mL

DAPI is a popular blue fluorescent DNA dye. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For live/intact cells, For fixed cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Colors

Blue

Size
Catalog #
price
Qty
1 mL
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Product Description

DAPI (4′,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate in powder form (catalog no. 40009).  DAPI dilactate is more water soluble than the dihydrochloride form of the dye.

DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Product Features:

  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λExEm (with DNA) = 358/461 nm
  • Yellow solution
  • Store at 4°C and protect from light
  • C22H27N5O6
  • MW: 457.49

Learn more about DAPI and our novel nuclear stains. We also offer antifade mounting media containing DAPI, in both hardset and wetset formulations.

DAPI Products

Product

Unit Size

Catalog Number

DAPI10 mg40011
DAPI in H2O, 10 mg/mL1 mL40043
DAPI, dilactate10 mg40009

References

n/a

 

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