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Donkey Anti-Human IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-human IgG (H+L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Secondary, Anti-Human Immunoglobulin

Clonality

Polyclonal

Host species

Donkey

Antibody reactivity (target)

Human IgG

Species reactivity

Human

Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Mouse, Rabbit, Rat, Sheep, Syrian hamster

Concentration

2 mg/mL

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Size
Catalog #
price
Qty
50 uL
0.25 mL
0.5 mL
1 mg
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Product Description

This is a highly cross-adsorbed donkey anti-human IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, horse, mouse, rabbit, rat, sheep, and Syrian hamster serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 6 bright and photostable CF® Dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Donkey Anti-Human IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.
CF®488A490/515 nm50 uL (100 ug)20074-1
0.5 mL (1 mg)20074
1 mg20074-1mg
CF®543541/560 nm50 uL (100 ug)20318-1
0.5 mL (1 mg)20318
1 mg20318-1mg
CF®594593/614 nm50 uL (100 ug)20075-1
0.5 mL (1 mg)20075
1 mg20075-1mg
CF®633630/650 nm50 uL (100 ug)20076-1
0.5 mL (1 mg)20076
1 mg20076-1mg
CF®680681/698 nm50 uL (100 ug)20278-1
0.25 mL (500 ug)20278
CF®770770/797 nm50 uL (100 ug)20279-1
0.25 mL (500 ug)20279

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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