Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Purified recombinant firefly luciferase enzyme is available commercially, and can be a useful positive control for luciferase assays. Firefly Luciferase Stabilizer is required when assaying purified recombinant firefly luciferase in the Passive Lysis Buffer provided in Biotium’s Firefly & Renilla Luciferase Single Tube Assay Kit (catalog no. 30081). We also offer the Firefly and Renilla Luciferase Single Tube Assay Kit for Purified Luciferase (catalog no. 30084), which includes Stabilizer along with the other Single Tube Assay components.
Stabilizer not required when using Passive Lysis Buffer with transfected cells or purified recombinant Renilla luciferase.