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Goat Anti-Mouse IgG (H+L)

Goat anti-mouse IgG (H+L) secondary antibody labeled with our superior CF® dyes and other labels.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Mouse IgG

Species reactivity

Mouse

Cross adsorption

Not cross-adsorbed

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates), 0.5 mg/mL (R-PE, APC conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, R-PE conjugates: PBS/2 mg/mL BSA/0.05% azide, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Catalog #
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50 uL
100 uL
200 uL
500 uL
0.5 mL
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1 mg
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Product Description

This is a goat anti-mouse IgG (H L) secondary antibody labeled with our superior CF® dyes.

  • Available in 18 bright and photostable CF® dyes
  • Alkaline phosphatase, APC, HRP, R-PE and biotin conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L)

ConjugationEx/EmSizeCatalog No.
CF®350347/448 nm50 uL (100 ug)20140-1
0.5 mL (1 mg)20140
1 mg (lyophilized)20140-1mg
CF®405S404/431 nm50 uL (100 ug)20080-1
0.5 mL (1 mg)20080
1 mg (lyophilized)20080-1mg
CF®405M408/452 nm50 uL (100 ug)20180-1
0.5 mL (1 mg)20180
1 mg (lyophilized)20180-1mg
CF®405L395/545 nm50 uL (100 ug)20408-50uL
0.5 mL (1 mg)20408-500uL
1 mg (lyophilized)20408-1mg
CF®488A490/515 nm50 uL (100 ug)20010-1
0.5 mL (1 mg)20010
1 mg (lyophilized)20010-1mg
CF®514516/548 nm50 uL (100 ug)20386-50uL
0.5 mL (1 mg)20386-500uL
1 mg (lyophilized)20386-1mg
CF®532527/558 nm50 uL (100 ug)20365-50uL
0.5 mL (1 mg)20365-500uL
1 mg (lyophilized)20365-1mg
CF®543541/560 nm50 uL (100 ug)20306-1
0.5 mL (1 mg)20306
1 mg (lyophilized)20306-1mg
CF®555555/565 nm50 uL (100 ug)20030-1
0.5 mL (1 mg)20030
1 mg (lyophilized)20030-1mg
CF®568562/583 nm50 uL (100 ug)20100-1
0.5 mL (1 mg)20100
1 mg (lyophilized)20100-1mg
CF®594593/614 nm50 uL (100 ug)20110-1
0.5 mL (1 mg)20110
1 mg (lyophilized)20110-1mg
CF®633630/650 nm50 uL (100 ug)20120-1
0.5 mL (1 mg)20120
1 mg (lyophilized)20120-1mg
CF®640R642/662 nm50 uL (100 ug)20197-1
0.5 mL (1 mg)20197
1 mg (lyophilized)20197-1mg
CF®647650/665 nm50 uL (100 ug)20040-1
0.5 mL (1 mg)20040
1 mg (lyophilized)20040-1mg
CF®660R663/682 nm50 uL (100 ug)20054-1
0.5 mL (1 mg)20054
1 mg (lyophilized)20054-1mg
CF®660C667/685 nm50 uL (100 ug)20050-1
0.5 mL (1 mg)20050
1 mg (lyophilized)20050-1mg
CF®750755/777 nm50 uL (100 ug)20070-1
0.5 mL (1 mg)20070
1 mg (lyophilized)20070-1mg
CF®790784/806 nm50 uL (100 ug)20378-50uL
R-PE496, 546, 565/578 nm200 uL (100 ug)20352-200uL
1 mL (500 ug)20352-1mL
APC650/660 nm100 uL (50 ug)20411-100uL
0.5 mL (250 ug)20411-0.5mL
BiotinN/A50 uL (100 ug)20183-1
0.5 mL (1 mg)20183
1 mg (lyophilized)20183-1mg
HRPN/A100 uL (100 ug)20400-100uL
1 mL (1 mg)20400-1mL
1 mg (lyophilized)20400-1mg
APN/A100 uL (100 ug)20464-100uL
1 mL (1 mg)20464-1mL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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