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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Mouse IgG

Species reactivity

Mouse

Cross adsorption

Bovine, Horse, Human, Rabbit, Swine

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Catalog #
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50 uL
100 uL
250 uL
0.25 mL
500 uL
0.5 mL
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1 mg
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Product Description

This is a highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, human, rabbit, and swine serum. This antibody is not cross-adsorbed against rat. For staining rat tissue, or co-staining with rat antibodies, we recommend using Goat Anti-Mouse IgG (H L), Highly Cross-Adsorbed (Min x Rat).

  • Highly-cross adsorbed for specific staining with minimal background
  • Available with 19 bright and photostable CF® Dyes
  • Biotin and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm50 uL (100 ug)20143-1CF®350 Features
0.5 mL (1 mg)20143
1 mg (lyophilized)20143-1mg
CF®405M408/452 nm50 uL (100 ug)20182-1CF®405M Features
0.5 mL (1 mg)20182
1 mg (lyophilized)20182-1mg
CF®430426/498 nm50 uL (100 ug)20459-50uLCF®430 Features
0.5 mL (1 mg)20459-500uL
1 mg (lyophilized)20459-1mg
CF®488A490/515 nm50 uL (100 ug)20018-1CF®488A Features
0.5 mL (1 mg)20018
1 mg (lyophilized)20018-1mg
CF®532527/558 nm50 uL (100 ug)20468-50uLCF®532 Features
0.5 mL (1 mg)20468-500uL
1 mg (lyophilized)20468-1mg
CF®543541/560 nm50 uL (100 ug)20299-1CF®543 Features
0.5 mL (1 mg)20299
1 mg (lyophilized)20299-1mg
CF®555555/565 nm50 uL (100 ug)20231-1CF®555 Features
0.5 mL (1 mg)20231
1 mg (lyophilized)20231-1mg
CF®568562/583 nm50 uL (100 ug)20101-1CF®568 Features
0.5 mL (1 mg)20101
1 mg (lyophilized)20101-1mg
CF®583R585/609 nm50 uL (100 ug)20904-50uLCF®583R Features
0.5 mL (1 mg)20904-500uL
CF®594593/614 nm50 uL (100 ug)20111-1CF®594 Features
0.5 mL (1 mg)20111
1 mg (lyophilized)20111-1mg
CF®633630/650 nm50 uL (100 ug)20121-1CF®633 Features
0.5 mL (1 mg)20121
1 mg (lyophilized)20121-1mg
CF®640R642/662 nm50 uL (100 ug)20175-1CF®640R Features
0.5 mL (1 mg)20175
1 mg (lyophilized)20175-1mg
CF®647650/665 nm50 uL (100 ug)20281-1CF®647 Features
0.5 mL (1 mg)20281
1 mg (lyophilized)20281-1mg
CF®660C667/685 nm50 uL (100 ug)20052-1CF®660C Features
0.5 mL (1 mg)20052
1 mg (lyophilized)20052-1mg
CF®680R680/701 nm50 uL (100 ug)20192-1CF®680 Features
0.25 mL (500 ug)20192
CF®680681/698 nm50 uL (100 ug)20065-1CF®680R Features
0.25 mL (500 ug)20065
CF®750755/777 nm50 uL (100 ug)20463-50uLCF®750 Features
250 uL (100 ug)20463-250uL
CF®770770/797 nm50 uL (100 ug)20077-1CF®770 Features
0.25 mL (500 ug)20077
CF®790784/806 nm50 uL (100 ug)20342-50uLCF®790 Features
BiotinN/A50 uL (100 ug)20184-1
0.5 mL (1 mg)20184
1 mg (lyophilized)20184-1mg
HRPN/A100 uL (100 ug)20401-100uL
1 mL (1 mg)20401-1mL
1 mg (lyophilized)20401-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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