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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

Highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Clonality

Polyclonal

Antibody type

Secondary

Concentration

2 mg/mL

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution

Species reactivity

Mouse

Secondary/tag antibody applications

Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Host species

Goat

Antibody reactivity (target)

Mouse IgG

Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Rabbit, Rat, Sheep, Syrian hamster

Conjugation
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Size
Catalog #
price
Qty
50 uL
0.5 mL
1 mg
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Product Description

This is a highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes and other labels. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat and sheep serum proteins.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 10 bright and photostable CF® Dyes
  • Suitable for western, immunofluorescence, and immunohistology

Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405S404/431 nm50 uL (100 ug)20830-50uLCF®405S Features
0.5 mL (1 mg)20830-500uL
1 mg (lyophilized)20830-1mg
CF®405M408/452 nm50 uL (100 ug)20340-50uLCF®405M Features
0.5 mL (1 mg)20340-500uL
1 mg (lyophilized)20340-1mg
CF®488A490/515 nm50 uL (100 ug)20302-1CF®488A Features
0.5 mL (1 mg)20302
1 mg (lyophilized)20302-1mg
CF®543541/560 nm50 uL (100 ug)20328-1CF®543 Features
0.5 mL (1 mg)20328
1 mg (lyophilized)20328-1mg
CF®568562/583 nm50 uL (100 ug)20301-1CF®568 Features
0.5 mL (1 mg)20301
1 mg (lyophilized)20301-1mg
CF®583R585/609 nm50 uL (100 ug)20903-50uLCF®583R Features
0.5 mL (1 mg)20903-500uL
CF®594593/614 nm50 uL (100 ug)20303-1CF®594 Features
0.5 mL (1 mg)20303
1 mg (lyophilized)20303-1mg
CF®633630/650 nm50 uL (100 ug)20341-50uLCF®633 Features
0.5 mL (1 mg)20341-500uL
1 mg (lyophilized)20341-1mg
CF®640R642/662 nm50 uL (100 ug)20304-1CF®640R Features
0.5 mL (1 mg)20304
1 mg (lyophilized)20304-1mg
CF®660C667/685 nm50 uL (100 ug)20368-50uLCF®660C Features
0.5 mL (1 mg)20368-500uL
1 mg (lyophilized)20368-1mg
To minimize crossreactivity, this antibody has been adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep serum.

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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