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Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-rabbit IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Rabbit IgG

Species reactivity

Rabbit

Cross adsorption

Human, Mouse, Rat

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Size
Catalog #
price
Qty
50 uL
100 uL
0.25 mL
500 uL
0.5 mL
1 mL
1 mg
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Product Description

This is a highly cross-adsorbed goat anti-rabbit IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against human, mouse, and rat serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 19 bright and photostable CF® Dyes
  • Biotin and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm50 uL (100 ug)20144-1CF®350 Features
0.5 mL (1 mg)20144
1 mg20144-1mg
CF®405M408/452 nm50 uL (100 ug)20373-50uLCF®405M Features
0.5 mL (1 mg)20373-500uL
1 mg20373-1mg
CF®430426/498 nm50 uL (100 ug)20460-50uLCF®430 Features
1 mg20460-1mg
0.5 mL (1 mg)20460-500uL
CF®488A490/515 nm50 uL (100 ug)20019-1CF®488A Features
0.5 mL (1 mg)20019
1 mg20019-1mg
CF®532527/558 nm50 uL (100 ug)20469-50uLCF®532 Features
1 mg20469-1mg
0.5 mL (1 mg)20469-500uL
CF®543541/560 nm50 uL (100 ug)20300-1CF®543 Features
0.5 mL (1 mg)20300
1 mg20300-1mg
CF®555555/565 nm50 uL (100 ug)20232-1CF®555 Features
0.5 mL (1 mg)20232
1 mg20232-1mg
CF®568562/583 nm50 uL (100 ug)20103-1CF®568 Features
0.5 mL (1 mg)20103
1 mg20103-1mg
CF®583R585/609 nm50 uL (100 ug)20905-50uLCF®583R Features
0.5 mL (1 mg)20905-500uL
CF®594593/614 nm50 uL (100 ug)20113-1CF®594 Features
0.5 mL (1 mg)20113
1 mg20113-1mg
CF®633630/650 nm50 uL (100 ug)20123-1CF®633 Features
0.5 mL (1 mg)20123
1 mg20123-1mg
CF®640R642/662 nm50 uL (100 ug)20176-1CF®640R Features
0.5 mL (1 mg)20176
1 mg20176-1mg
CF®647650/665 nm50 uL (100 ug)20282-1CF®647 Features
0.5 mL (1 mg)20282
1 mg20282-1mg
CF®660C667/685 nm50 uL (100 ug)20369-50uLCF®660C Features
0.5 mL (1 mg)20369-500uL
1 mg20369-1mg
CF®680681/698 nm50 uL (100 ug)20067-1CF®680 Features
0.25 mL (500 ug)20067
CF®680R680/701 nm50 uL (100 ug)20193-1CF®680R Features
0.25 mL (500 ug)20193
CF®770770/797 nm50 uL (100 ug)20078-1CF®770 Features
0.25 mL (500 ug)20078
CF®790784/806 nm50 uL (100 ug)20343-50uLCF®790 Features
BiotinN/A50 uL (100 ug)20186-1
0.5 mL (1 mg)20186
1 mg20186-1mg
HRPN/A100 uL (100 ug)20403-100uL
1 mL (1 mg)20403-1mL
1 mg20403-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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