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Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-rat IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Rat IgG

Species reactivity

Rat

Cross adsorption

Bovine, Horse, Human, Rabbit

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates), 0.5 mg/mL (R-PE, APC conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, R-PE conjugates: PBS/2 mg/mL BSA/0.05% azide, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Size
Catalog #
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50 uL
100 uL
200 uL
0.25 mL
500 uL
0.5 mL
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1 mg
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Product Description

This is a highly cross-adsorbed goat anti-rat IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, human, and rabbit serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 14 bright and photostable CF® Dyes
  • APC, HRP, and R-PE conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm0.5 mL (1 mg)20147CF®350 Features
1 mg20147-1mg
50 uL (100 ug)20147-1
CF®405M408/452 nm0.5 mL (1 mg)20374-500uLCF®405M Features
1 mg20374-1mg
50 uL (100 ug)20374-50uL
CF®488A490/515 nm0.5 mL (1 mg)20023CF®488A Features
1 mg20023-1mg
50 uL (100 ug)20023-1
CF®543541/560 nm0.5 mL (1 mg)20321CF®543 Features
1 mg20321-1mg
50 uL (100 ug)20321-1
CF®555555/565 nm0.5 mL (1 mg)20233CF®555 Features
1 mg20233-1mg
50 uL (100 ug)20233-1
CF®568562/583 nm0.5 mL (1 mg)20096CF®568 Features
1 mg20096-1mg
50 uL (100 ug)20096-1
CF®583R585/609 nm50 uL (100 ug)20906-50uLCF®583R Features
0.5 mL (1 mg)20906-500uL
CF®594593/614 nm0.5 mL (1 mg)20155CF®594 Features
1 mg20155-1mg
50 uL (100 ug)20155-1
CF®633630/650 nm0.5 mL (1 mg)20133CF®633 Features
1 mg20133-1mg
50 uL (100 ug)20133-1
CF®640R642/662 nm0.5 mL (1 mg)20088CF®640R Features
1 mg20088-1mg
50 uL (100 ug)20088-1
CF®647650/665 nm0.5 mL (1 mg)20283CF®647 Features
1 mg20283-1mg
50 uL (100 ug)20283-1
CF®660C667/685 nm0.5 mL (1 mg)20370-500uLCF®660C Features
1 mg20370-1mg
50 uL (100 ug)20370-50uL
CF®680681/698 nm0.25 mL20069CF®680 Features
50 uL (100 ug)20069-1
CF®770770/797 nm0.25 mL20383-250uLCF®770 Features
50 uL (100 ug)20383-50uL
R-PE496, 546, 565/578 nm200 uL (100 ug)20354-200uL
1 mL (500 ug)20354-1mL
APC650/660 nm100 uL (50 ug)20413-100uL
0.5 mL (250 ug)20413-500uL
HRPN/A100 uL (100 ug)20406-100uL
1 mL (1 mg)20406-1mL
1 mg20406-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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