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Hoechst 33258, Pentahydrate

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For live/intact cells, For fixed cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue

CAS number

23491-45-4

Size
Catalog #
price
Qty
100 mg
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Product Description

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

  • λExEm (with DNA) = 352/461 nm
  • Pale yellow solid soluble in water up to 2% (w/v)
  • Store at -20°C and protect from light
  • C25H27Cl3N6O.5H2O
  • MW: 623.96
  • [23491-45-4]

Learn more about Hoechst and our other novel nuclear stains.

Hoechst Products

Product

Unit Size

Catalog Number

Hoechst 33258, 10 mg/mL in H2O10 mL40044
Hoechst 33258, pentahydrate100 mg40045
Hoechst 33342, 10 mg/mL in H2O10 mL40046
Hoechst 33342, trihydrochloride trihydrate100 mg40047

References

n/a

 

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