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Hoechst 33342, 10 mg/mL in H2O

For ease of use and safer handling, we offer both Hoechst 33258 and Hoechst 33342 dissolved in H2O at 10 mg/mL. Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains.

Product Attributes

CAS number

23491-52-3

Cellular localization

Nucleus

For live or fixed cells

For live/intact cells, For fixed cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue

Certificates of Analysis (COA)

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10 mL
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Product Description

For ease of use and safer handling, we offer both Hoechst 33258 and Hoechst 33342 dissolved in H2O at 10 mg/mL. Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258, but either dye can be used to stain live or fixed cell in buffer or medium at 1 ug/mL, with no wash step required.

  • λExEm (with DNA) = 350/461 nm
  • Light yellow solution
  • Store at 4°C and protect from light
  • C27H31Cl3N6O
  • MW: 561.93
  • [23491-52-3]

Learn more about Hoechst and our other novel nuclear stains.

Hoechst Products

Product

Unit Size

Catalog Number

Hoechst 33258, pentahydrate100 mg40045
Hoechst 33258, 10 mg/mL in H2O10 mL40044
Hoechst 33342, trihydrochloride trihydrate100 mg40047
Hoechst 33342, 10 mg/mL in H2O10 mL40046

References

n/a

 

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