Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Hoechst 33342, Trihydrochloride Trihydrate

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.

Product Attributes

CAS number

23491-52-3

Cellular localization

Nucleus

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue

Excitation/Emission

350/461 nm (with DNA)

Size
Catalog #
price
Qty
100 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O (catalog no. 40046).

Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Hoechst Features:

  • λExEm = 350/461 nm (with DNA)
  • Pale yellow solid soluble in water up to 2% (w/v)
  • Store at 4°C and protect from light
  • C27H31Cl3N6O.3H2O
  • MW: 615.98
  • [23491-52-3]

 

Learn more about Hoechst and our other novel nuclear stains.

Hoechst Products

Product

Unit Size

Catalog Number

Hoechst 33258, 10 mg/mL in H2O10 mL40044
Hoechst 33258, pentahydrate100 mg40045
Hoechst 33342, 10 mg/mL in H2O10 mL40046
Hoechst 33342, trihydrochloride trihydrate100 mg40047

References

n/a

 

You may also like…