Support & Resources

+1

Biotin Monoclonal Mouse Antibody (3D6.6)

Monoclonal mouse anti-biotin antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Tag Antibody

Antibody reactivity (target)

Biotin

Host species

Mouse

Clonality

Monoclonal

Clone

3D6.6

Isotype

IgG1, kappa

Concentration

2 mg/mL

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide

Storage Conditions

Store at -10 to -35 °C, Protect from light

Secondary/tag antibody applications

Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Size
Catalog #
price
Qty
50 uL
0.25 mL
Clear selection
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

This is a monoclonal mouse anti-biotin antibody labeled with our superior CF® Dyes. The antibody is useful for detecting biotin conjugated to antibodies or other proteins.

  • Available in 8 bright and photostable CF® Dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

See our full selection of anti-tag and anti-hapten antibody conjugates.

Note: Conjugates of blue fluorescent dyes like CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Monoclonal Mouse Anti-Biotin

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405S404/431 nm50 uL (100 ug)20203-1CF®405S Features
0.25 mL (500 ug)20203
CF®488A490/515 nm50 uL (100 ug)20204-1CF®488A Features
0.25 mL (500 ug)20204
CF®568562/584 nm50 uL (100 ug)20502-1CF®568 Features
0.25 mL (500 ug)20502
CF®594593/614 nm50 uL (100 ug)20205-1CF®594 Features
0.25 mL (500 ug)20205
CF®633630/650 nm50 uL (100 ug)20206-1CF®633 Features
0.25 mL (500 ug)20206
CF®640R642/662 nm50 uL (100 ug)20207-1CF®640R Features
0.25 mL (500 ug)20207
CF®750755/777 nm50 uL (100 ug)20501-50uLCF®750 Features
0.25 mL (500 ug)20501-250uL
CF®770770/797 nm50 uL (100 ug)20367-50uLCF®770 Features
0.25 mL (500 ug)20367-250uL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

You may also like…