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GFP Monoclonal Mouse Antibody (9F9.F9)

Monoclonal mouse anti-GFP antibody labeled with our superior CF® dyes.

Product Attributes

Antibody type

Tag Antibody

Antibody reactivity (target)

GFP

Host species

Mouse

Clonality

Monoclonal

Clone

9F9.F9

Isotype

IgG1, kappa

Immunogen

Fusion protein corresponding to Aequorea victoria full length GFP, aa 1-246

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide

Concentration

1 mg/mL

Secondary/tag antibody applications

Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Size
Catalog #
price
Qty
100 uL
0.1 mL
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Product Description

This is a monoclonal mouse anti-GFP (green fluorescent protein) antibody labeled with our superior CF® dyes.

  • Available in 9 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

See our full selection of anti-tag and anti-hapten antibody conjugates.

GFP Monoclonal Mouse Antibody

ConjugationEx/EmSizeCatalog No.
CF®488A490/515 nm100 uL (100 ug)20215
CF®568562/583 nm100 uL (100 ug)20480
CF®594593/614 nm100 uL (100 ug)20216
CF®633630/650 nm100 uL (100 ug)20217
CF®640R642/662 nm100 uL (100 ug)20218
CF®660R663/682 nm100 uL (100 ug)20481
CF®680681/698 nm100 uL (100 ug)20219
CF®680R680/701 nm100 uL (100 ug)20482
CF®770770/797 nm100 uL (100 ug)20220

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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