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NucSpot® 470 Nuclear Stain, 1000X in DMSO

A cell membrane-impermeant, nuclear-specific green fluorescent DNA stain.

Product Attributes

Probe cellular localization


For live or fixed cells

For fixed cells

Assay type/options

DNA content/cell cycle profiling by flow cytometry, Live/dead discrimination, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining

Detection method/readout

Fluorescence microscopy, Flow cytometry

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)



Storage Conditions

Store at 2 to 8 °C


460/546 nm (with DNA)

Catalog #
20 uL
200 uL
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Supporting Documents

Product Description

NucSpot® 470 is a cell membrane-impermeant green fluorescent DNA stain for nuclear counterstaining of fixed cells, or selective staining of dead cells.

  • Nuclear-specific green counterstain for fixed cells
  • Selectively stains dead cells in live culture
  • Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells
  • No wash required
  • Abs/Em = 460/546 nm for FITC channel
  • Excellent match for blue LED excitation sources

NucSpot® 470 is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO®, TO-PRO®, or SYTOX® dyes stain both the nucleus and cytoplasm, NucSpot® 470 specifically stains the nucleus of fixed and permeabilized cells, as well as DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without the need for RNase-treatment, unlike propidium iodide. It also can be used to selectively stain dead cells in living cultures. The dye has green fluorescence (Ex/Em 460/546 nm) that can be imaged using standard settings for FITC, and is an excellent match for instruments with blue LED excitation sources.

NucSpot® 470 is nuclear-specific in dead or fixed/permeabilized yeast cells. In gram-negative bacteria it selectively stains dead cells, while in gram-positive bacteria it may stain live cells. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Learn more about our full selection of Cellular Stains for the nucleus and other organelles.

TOTO, TO-PRO, and SYTOX are registered trademarks of Thermo Fisher Scientific.



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