Support & Resources


ViaFluor® SE Cell Proliferation Kits

ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

Product Attributes

Dye Color

ViaFluor® CFSE, ViaFluor® 405, ViaFluor® 488

Apoptosis/viability marker

All cell stain

Cellular localization

Nucleus & cytoplasm

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Cell division tracking, Extended staining (several days to weeks), Real-time imaging

Cell permeability

Membrane permeant


Blue, Green

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Dye Color
Catalog #
Trial size (1 vial)
Set of 10 vials
Clear selection
Add to wishlist
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

ViaFluor®  SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. Labeling is covalent, so it withstands fixation and permeabilization for subsequent immunostaining.


  • Non-toxic dyes covalently label cell cytoplasm
  • Track cell proliferation in vivo or in vitro by dye dilution in flow cytometry
  • Long term imaging of cell morphology or co-cultures by microscopy
  • Staining can be fixed and permeabilized for immunofluorescence
  • Violet ViaFluor® 405 offers excellent performance at lower cost
  • Green ViaFluor® 488 SE is much less toxic than CFSE

The membrane permeable compound is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to releases the fluorescent amine-reactive dye. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away.  Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining.  Thus, cell proliferation dyes can be used to track multiple cell divisions of cells grown in culture or injected in vivo after labeling with the ViaFluor® SE dye.

All three ViaFluor® SE dyes can stain gram-positive bacteria, but not gram-negative bacteria. ViaFluor® CFSE stains the cytoplasm in yeast, but ViaFluor® 405 & ViaFluor® 488 stain the yeast cell periphery. See our Cellular Stains Table for more information on how our dyes stain various organisms.

ViaFluor® SE dyes also can be used for imaging cell morphology or identifying cells in co-culture by microscopy. Because the staining is non-toxic and well-retained, it can be used for imaging  live cells over time. See our Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture.

Kit Components:

  • 10 lyophilized dye vials (full size kits)
  • 1 lyophilized dye vial (trial size kits)
  • 1 vial of anhydrous DMSO for preparing stock solutions

When used at 1 uM in 1 mL of cells, at a cell density of 1 x 106 cells mL, each dye vial can be used for 90-100 labelings; the final number of assays that can be performed per kit depends on the dye concentration used (see product protocol under downloads).

ViaFluor® kits are available with 3 dye options:

  • ViaFluor® CFSE (also known as CFDA-SE) can be detected in the FITC channel.
  • ViaFluor® 488 SE, an improved alternative to CFSE; detected in the FITC channel.
  • ViaFluor® 405 SE for the violet laser; can be detected in the Pacific Blue® channel.

Please note that ViaFluor® 568 (Cat. # 30080) has been discontinued as of 12-15-17.

Cell Division Tracking Dyes

Catalog No.

Ex/Em (nm)

Flow detection channel


ViaFluor® 405 Cell Proliferation Kit30068/30068-T408/452 Pacific Blue®
  • Track cell division by dye dilution using flow cytometry

  • ViaFluor® 488 is a unique, improved green dye to replace CFSE

  • ViaFluor® 405 works as well as CellTrace™ Violet
ViaFluor® 488 Cell Proliferation Kit30086/30086-T493/532FITC
ViaFluor® CFSE Cell Proliferation Kit30050495/519FITC
Pacific Blue is a registered trademark of Thermo Fisher Scientific.


ViaFluor® 405, non-toxicicity:
Kamal, M., Saremi, S., Klotz, R. et al. (2019). PIC&RUN: An integrated assay for the detection and retrieval of single viable circulating tumor cells. Sci Rep 9, 17470. doi:10.1038/s41598-019-53899-4.


You may also like…