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qPCR Reagents

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    dNTP Mix, 25 mM Each

    From: $195 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM Each

    From: $195 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    EvaGreen® Dye, 2000X in DMSO

    From: $181 Sizes: 50 uLCatalog #: 31019

    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

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    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

     

    Fast EvaGreen® qPCR Master Mix

    From: $68 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31003, 31003-1, 31003-2, - 31003-TView allHide

    Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

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    Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

     

    Fast Plus EvaGreen® qPCR Master Mix

    From: $78 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31014, 31015, 31020, 31014-1, 31014-2, 31014-T, 31015-1, 31015-2, 31015-T, 31020-1, 31020-2, - 31020-TView allHide

    Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium's PCR master mix delivers superior performance even with the most challenging samples tested.

    Reference dye
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    Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium's PCR master mix delivers superior performance even with the most challenging samples tested.

     

    Fast Probe Master Mix

    From: $85 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31005, 31016, 31005-1, 31005-2, 31005-T, 31016-1, 31016-2, - 31016-TView allHide

    Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan® probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq.

    Reference dye
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    Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan® probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq.

     

    HotStart Polymerase Modification Kit

    From: $200 Sizes: Trial size, 1 kitCatalog #: 29054, - 29054-TView allHide

    The HotStart Polymerase Modification Kit provides an easy way to reversibly modify lysine residues of thermostable DNA polymerase, rendering the enzyme inactive. The modification is reversed after heating to >90°C. HotStart modification of DNA polymerase for PCR prevents amplification of non-specific PCR products due to low stringency annealing of primers at low temperature during reaction assembly.

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    The HotStart Polymerase Modification Kit provides an easy way to reversibly modify lysine residues of thermostable DNA polymerase, rendering the enzyme inactive. The modification is reversed after heating to >90°C. HotStart modification of DNA polymerase for PCR prevents amplification of non-specific PCR products due to low stringency annealing of primers at low temperature during reaction assembly.

     

    ROX Reference Dye, 25 uM in TE Buffer

    From: $135 Sizes: 5 x 1 mLCatalog #: 29052

    ROX reference dye is used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions. The dye is supplied as a 25 uM solution in 10 mM Tris-HCL (pH 8.6), 0.1 mM EDTA, and 0.01% Tween-20.

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    ROX reference dye is used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions. The dye is supplied as a 25 uM solution in 10 mM Tris-HCL (pH 8.6), 0.1 mM EDTA, and 0.01% Tween-20.

     

    Thiazole Green, 10,000X in DMSO

    Sizes: 0.5 mL, 1 mLCatalog #: 40086-0.5mL, - 40086-1mLView allHide

    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

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    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

     

    WarmStart Enzyme Modification Kit

    From: $200 Sizes: Trial size, 1 kitCatalog #: 29053, - 29053-TView allHide

    The WarmStart™ Enzyme Modification Kit allows temperature control of enzyme activity to be applied to non-thermostable enzymes like reverse transcriptase. Similar to our HotStart polymerase modification reagent (catalog no. 29054), the enzyme is inactivated by modification of lysine residues with a novel chemical modifier. While HotStart modification is reversed by heating to 90°C or higher, WarmStart™ protein activity is restored by heating to 45°C or higher.

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    The WarmStart™ Enzyme Modification Kit allows temperature control of enzyme activity to be applied to non-thermostable enzymes like reverse transcriptase. Similar to our HotStart polymerase modification reagent (catalog no. 29054), the enzyme is inactivated by modification of lysine residues with a novel chemical modifier. While HotStart modification is reversed by heating to 90°C or higher, WarmStart™ protein activity is restored by heating to 45°C or higher.