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As with all fluorescence based probes, bleaching will occur over time. How long you can view NucView® staining under the microscope depends on several factors including the initial signal strength and the intensity of the excitation source. Some users have reported monitoring NucView® for 4-5 days. You can see a time-lapse video of NucView® staining over 24 hours here:

https://biotium.com/technology/nucview-caspase-3-substrates/

Yes. NucView® Caspase-3 Substrates are compatible with flow cytometers and other instruments with the appropriate excitation and emission settings.

The substrates are very stable. Some users have reported performing time course assays with NucView® 488 Caspase-3 Substrate for 4-5 days.

Yes. We recommend a gentle fix in 2-4% paraformaldehyde for 10-15 minutes. Over-fixing can cause the signal to decrease. NucView® 488 staining can withstand permeabilization with 0.1% Triton X-100. Methanol fixation is not recommended.

The NucView® caspase-3 substrates are activity-dependent i.e. require active caspase-3 enzyme. In dead, fixed and preserved cells or tissue sections, there are no active caspases and hence these substrates cannot be used. We also offer TUNEL assay kits that are suitable for apoptosis detection in fixed cells and tissues. The kits employ dUTPs conjugated to our exceptionally bright and photostable CF® dyes for single-step fluorescent TUNEL labeling of DNA strand breaks, a hallmark of apoptotic cells, and are suitable for analysis by fluorescence microscopy or flow cytometry.

Technology: NucView® Caspase-3 Substrates

 
Biotium's fluorogenic substrates for following caspase-3 activity in whole cells and in real-time

Product:Ac-IETD-R110

Sizes: 5 mgCatalog #: 10207

We currently offer NucView® in three dye colors: green (our original NucView® 488), blue (NucView® 405), and orange (NucView® 530). Other colors of NucView® are in development.

Our NucView® substrates detect cleavage by caspases 3 and 7. We do not currently have NucView® substrates for other caspases.

Product:DASPEI

Sizes: 100 mgCatalog #: 70018

The NucView® 488 Assay Kit for Live Cells (30029) contains NucView® 488 substrate at 0.2 mM in DMSO and caspase-3 inhibitor Ac-DEVD-CHO. The final DMSO concentration in the kit assay can be fairly high, which is undesirable for sensitive cell types. Because of this, we offer NucView® 488 Caspase-3 Substrate at 1 mM in DMSO (10402) and at 1 mM in PBS (10403), so customers can control the amount of DMSO in their assay. Note that in non-DMSO sensitive cell types, adding DMSO during the substrate incubation can enhance NucView® staining.

We also offer blue fluorogenic NucView® 405 in DMSO (10405) and PBS (10407), and orange fluorogenic NucView® 530 in DMSO (10406) and PBS (10408).

Product:Z-AAD-R110

Sizes: 5 mgCatalog #: 10211
Happy anniversary, NucView®! A decade of real-time apoptosis detection
Happy anniversary, NucView®! A decade of real-time apoptosis detection
NucView® technology: A decade of real-time apoptosis detection More than a decade after the launch of our NucView® caspase-3 substrate, Biotium ...

DEVD-CHO is a reversible inhibitor and may not sufficiently block caspase-3 activity. Adding an irreversible inhibitor like DEVD-FMK at the beginning of the experiment (before or at the time of apoptosis induction) may more effectively inhibit caspase activity.

Product:CD81 / TAPA-1 Monoclonal Mouse Antibody (1.3.3.22)

Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNCAP0391-100, BNCAP0391-500, BNCA0391-250, BNCB0391-100, BNCB0391-500, BNC050391-100, BNC050391-500, BNC040391-100, BNC040391-500, BNC880391-100, BNC880391-500, BNC430391-100, BNC430391-500, BNC550391-100, BNC550391-500, BNC680391-100, BNC680391-500, BNC940391-100, BNC940391-500, BNC400391-100, BNC400391-500, BNC470391-100, BNC470391-500, BNC610391-100, BNC610391-500, BNC800391-100, BNC800391-500, BNC810391-100, BNC810391-500, BNC700391-100, BNC700391-500, BNCH0391-100, BNCH0391-500, BNCP0391-250, BNUM0391-50, BNUB0391-100, BNUB0391-500, - BNCR0391-250View allHide

Page: Product Videos and Presentations

 
Video from Essen Bioscience featuring NucView® 488 Caspase 3 Substrate Cancer Cell Apoptosis ...

Product:CD95 Monoclonal Mouse Antibody (B-R18)

From: $138 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC050305-100, BNC400305-100, BNC470305-100, BNC550305-100, BNC680305-100, BNC700305-100, BNC800305-100, BNC880305-100, BNC940305-100, BNCAP0305-100, BNCB0305-100, BNCH0305-100, BNUB0305-100, BNCA0305-250, BNCP0305-250, BNCR0305-250, BNC050305-500, BNC400305-500, BNC470305-500, BNC550305-500, BNC680305-500, BNC700305-500, BNC800305-500, BNC880305-500, BNC940305-500, BNCAP0305-500, BNCB0305-500, BNUB0305-500, - BNUM0305-50View allHide

Product:Cytochrome c Monoclonal Mouse Antibody (CYCS/1010)

From: $157 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC041010-100, BNC041010-500, BNC051010-100, BNC051010-500, BNC401010-100, BNC401010-500, BNC431010-100, BNC431010-500, BNC471010-100, BNC471010-500, BNC551010-100, BNC551010-500, BNC611010-100, BNC611010-500, BNC681010-100, BNC681010-500, BNC701010-100, BNC701010-500, BNC801010-100, BNC801010-500, BNC811010-100, BNC811010-500, BNC881010-100, BNC881010-500, BNC941010-100, BNC941010-500, BNCA1010-250, BNCAP1010-100, BNCAP1010-500, BNCB1010-100, BNCB1010-500, BNCH1010-100, BNCH1010-500, BNCP1010-250, BNCR1010-250, BNUB1010-100, BNUB1010-500, - BNUM1010-50View allHide

Product:Cytochrome c Monoclonal Mouse Antibody (CTC05)

From: $157 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC040887-100, BNC040887-500, BNC050887-100, BNC050887-500, BNC400887-100, BNC400887-500, BNC430887-100, BNC430887-500, BNC470887-100, BNC470887-500, BNC550887-100, BNC550887-500, BNC610887-100, BNC610887-500, BNC680887-100, BNC680887-500, BNC700887-100, BNC700887-500, BNC800887-100, BNC800887-500, BNC810887-100, BNC810887-500, BNC880887-100, BNC880887-500, BNC940887-100, BNC940887-500, BNCA0887-250, BNCAP0887-100, BNCAP0887-500, BNCB0887-100, BNCB0887-500, BNCH0887-100, BNCH0887-500, BNCP0887-250, BNCR0887-250, BNUB0887-100, BNUB0887-500, - BNUM0887-50View allHide