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Product:MALT-1 Monoclonal Mouse Antibody (MT1/410)

From: $138 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC040410-100, BNC050410-100, BNC400410-100, BNC430410-100, BNC470410-100, BNC550410-100, BNC610410-100, BNC680410-100, BNC700410-100, BNC800410-100, BNC810410-100, BNC880410-100, BNC940410-100, BNCAP0410-100, BNCB0410-100, BNCH0410-100, BNUB0410-100, BNCA0410-250, BNCP0410-250, BNCR0410-250, BNC040410-500, BNC050410-500, BNC400410-500, BNC430410-500, BNC470410-500, BNC550410-500, BNC610410-500, BNC680410-500, BNC700410-500, BNC800410-500, BNC810410-500, BNC880410-500, BNC940410-500, BNCAP0410-500, BNCB0410-500, BNCH0410-500, BNUB0410-500, - BNUM0410-50View allHide

Page: Careers

 
Innovative dyes for science. Expert solutions for scientists. Biotium is a leading life science ...

There are usually three aspects to dye stability: 1) chemical stability of the dye core structure; 2) stability of the reactive group; and 3) photostability of the dye.

Chemical stability of the dye core structure:
This refers to resistance of the dye core structure to decomposition caused by factors other than photo-bleaching. These factors may include temperature, pH and incompatibility with other chemicals in the medium. This type of stability information is most useful for estimating the shelf-life of the dye that is already covalently attached to another molecule (e.g., an antibody), or for assessing the chemical compatibility of the dye in certain applications. CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes, all of which are known to have excellent chemical stability. In general, CF® dyes are far more stable than the antibodies they label. Thus, if a CF®-labeled antibody loses activity during storage, the problem is not likely to be caused by the dye. CF® dyes are also stable enough for labeled nucleic acids to be used in PCR or nucleic acid hybridization, where high temperature is involved.

Stability of the reactive group:
Reactive CF® dyes comprise a reactive group used in bioconjugation. Among the various reactive groups, only amine-reactive succinimidyl ester (SE) and thiol-reactive maleimide groups are unstable because the small amount of moisture trapped in or leaked into the packaging vials can cause hydrolysis of the reactive groups over time. The SE group, in particular, is susceptible to degradation. Thus, in order to slow degradation, CF® dyes comprising these reactive groups must be stored at -20°C under anhydrous conditions. Furthermore, stock solutions of the dyes must be made using dry solvents, such as anhydrous DMSO. One advantage of CF® dye SE products over other commercial dyes is their relatively high stability. Normally, an SE group can be derived from either an aliphatic or an aromatic carboxylic acid group, but an aliphatic carboxylic group tends to result in a more stable SE, offering higher resistance to hydrolysis and thus better labeling efficiency. All of the CF® SE dyes have their SE groups derived from aliphatic carboxylic acid groups, unlike many of the Alexa Fluor SE dyes, which are prepared from aromatic carboxylic acid groups.

Photostability:
This refers to the dye’s ability to withstand photobleaching. For most dyes, photostability is not a major problem for routine handling under ambient light or for applications, such as flow cytometry and Western blotting, where the dyes are only briefly exposed to light. However, for microscopy, especially for confocal microscopy, where the dyes may be subject to intense illumination for an extended period of time, photobleaching can be a major concern. Similar to the photostability of other fluorescent dyes, both the dye core structure and the structure-modifying groups attached to it play a role in the photostability of CF® dyes. CF® dyes bear the core structure of rhodamine, cyanine, pyrene or coumarin dyes; among the four types of core structures, rhodamine core is the most photostable, followed by cyanine and then by pyrene and coumarin cores. The structure-modifying groups and the way they are attached to the dye cores are a key innovative aspect of CF® dye technologies that contributes to the superior photostability of CF® dyes over that of other commercial dyes. In general, rhodamine-based CF® dyes, whose wavelengths range from visible to the near-IR region, offer the best photostability, making the dye ideal for microscopy applications.

12 years later: Viability PCR using PMA and PMAxx™ dyes
12 years later: Viability PCR using PMA and PMAxx™ dyes
Biotium developed PMA dye for viability PCR 12 years ago, an advance that has spawned countless publications and contributed to basic research, ...

Product:N-Cadherin / Cadherin-2 / CD325 Monoclonal Mouse Antibody (8C11)

Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNCAP1426-100, BNCAP1426-500, BNCA1426-250, BNCB1426-100, BNCB1426-500, BNC051426-100, BNC051426-500, BNC041426-100, BNC041426-500, BNC881426-100, BNC881426-500, BNC431426-100, BNC431426-500, BNC551426-100, BNC551426-500, BNC681426-100, BNC681426-500, BNC941426-100, BNC941426-500, BNC401426-100, BNC401426-500, BNC471426-100, BNC471426-500, BNC611426-100, BNC611426-500, BNC801426-100, BNC801426-500, BNC811426-100, BNC811426-500, BNC701426-100, BNC701426-500, BNCH1426-100, BNCH1426-500, BNCP1426-250, BNUM1426-50, BNUB1426-100, BNUB1426-500, - BNCR1426-250View allHide

Product:N-Cadherin / Cadherin-2 / CD325 Monoclonal Mouse Antibody (13A9)

Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNCAP1425-100, BNCAP1425-500, BNCA1425-250, BNCB1425-100, BNCB1425-500, BNC051425-100, BNC051425-500, BNC041425-100, BNC041425-500, BNC881425-100, BNC881425-500, BNC431425-100, BNC431425-500, BNC551425-100, BNC551425-500, BNC681425-100, BNC681425-500, BNC941425-100, BNC941425-500, BNC401425-100, BNC401425-500, BNC471425-100, BNC471425-500, BNC611425-100, BNC611425-500, BNC801425-100, BNC801425-500, BNC811425-100, BNC811425-500, BNC701425-100, BNC701425-500, BNCH1425-100, BNCH1425-500, BNCP1425-250, BNUM1425-50, BNUB1425-100, BNUB1425-500, - BNCR1425-250View allHide

Product:CD53 Monoclonal Mouse Antibody (161-2)

From: $138 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC050324-100, BNC400324-100, BNC430324-100, BNC470324-100, BNC550324-100, BNC610324-100, BNC680324-100, BNC700324-100, BNC800324-100, BNC880324-100, BNC940324-100, BNCAP0324-100, BNCB0324-100, BNCH0324-100, BNUB0324-100, BNCA0324-250, BNCP0324-250, BNCR0324-250, BNC050324-500, BNC400324-500, BNC430324-500, BNC470324-500, BNC550324-500, BNC610324-500, BNC680324-500, BNC700324-500, BNC800324-500, BNC810324-500, BNC880324-500, BNC940324-500, BNCAP0324-500, BNCB0324-500, BNCH0324-500, BNUB0324-500, - BNUM0324-50View allHide

Product:CD53 Monoclonal Mouse Antibody (63-5A3)

From: $138 Sizes: 50 uL, 100 uL, 250 uL, 500 uLCatalog #: BNC040356-100, BNC050356-100, BNC400356-100, BNC430356-100, BNC470356-100, BNC550356-100, BNC610356-100, BNC680356-100, BNC700356-100, BNC800356-100, BNC810356-100, BNC940356-100, BNCAP0356-100, BNCB0356-100, BNCH0356-100, BNUB0356-100, BNCA0356-250, BNCR0356-250, BNC040356-500, BNC050356-500, BNC400356-500, BNC430356-500, BNC470356-500, BNC550356-500, BNC610356-500, BNC680356-500, BNC700356-500, BNC800356-500, BNC810356-500, BNC880356-500, BNC940356-500, BNCB0356-500, BNCH0356-500, BNUB0356-500, - BNUM0356-50View allHide

Product:MCB Glutathione Detection Kit

From: $333 Sizes: 100 assaysCatalog #: 30019

Product:JC-1 Mitochondrial Membrane Potential Detection Kit

From: $72 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30001, - 30001-TView allHide

Product:Annexin V Conjugates

Sizes: 100 uL, 500 uL, 0.5 mLCatalog #: 29001, 29002, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29057-500uL, - 29083View allHide

The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.