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The Full Spectrum

A Biotium Blog

Protocols Library
Protocols Library
Antibody Staining Protocols Considerations for Immunofluorescence Immunofluorescence Staining ...
Antibody Staining Protocols Considerations for Immunofluorescence Immunofluorescence Staining for Microscopy Cell Surface Staining for Flow Cytometry Intracellular Staining for Flow Cytometry ...
Tech Tip: Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits
Tech Tip: Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits
Introduction Biotium’s Tyramide Amplification Kits are powerful tools to achieve immunofluorescence staining of cells or tissues with exceptional ...
Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence
Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence
CellBrite™ Cytoplasmic Membrane Dyes are membrane dyes that are useful for visualizing cell outlines. However, because they are ...
Tech Tip: Five steps to successful cell membrane staining & imaging
Tech Tip: Five steps to successful cell membrane staining & imaging
Tips for success with CellBrite™, CellBrite™ Fix, and MemBrite™ Fix membrane and cell surface stains Membrane and cell ...
Tech Tip: Cell Surface Stains for Live & Fixed Cells
Tech Tip: Cell Surface Stains for Live & Fixed Cells
Membrane staining is very useful in defining cell boundaries in multicolor imaging studies. Researchers are ever looking for suitable dyes to use ...
Tech Tip: Eight things to keep in mind for optimal gel staining with GelRed® and GelGreen®
Tech Tip: Eight things to keep in mind for optimal gel staining with GelRed® and GelGreen®
The most common use of GelRed® and GelGreen® nucleic acid stains is in pre-cast agarose gels, where the dyes are added to molten agarose during ...
Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture
Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture
ViaFluor®  SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cytoplasm and intracellular compartments. ...
Tech Tip: Imaging Bacteria Using Agarose Pads
Tech Tip: Imaging Bacteria Using Agarose Pads
Tech tip: Imaging bacteria using the agarose pad technique Bacteria can be more difficult to image than other cell types because they are small, ...
Tech Tip: Battling Tissue Autofluorescence
Tech Tip: Battling Tissue Autofluorescence
Immunofluorescence staining offers a variety of advantages compared to colorimetric immunocytochemistry, most importantly, simultaneous ...
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Cross-talk and specificity in multi-color imaging experiments Cross-talk between fluorescence channels is an important concern for multi-color ...
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Immunofluorescence staining (IF) can be performed in two ways, by direct or indirect detection. Direct IF uses a dye-conjugated antibody to stain ...
Protocol: Immunofluorescence Staining of Cells for Microscopy
Protocol: Immunofluorescence Staining of Cells for Microscopy
There are many variations on IF protocols, and steps may need to be optimized for different targets or applications. Some epitopes may require ...
Protocol: Cell Surface Antibody Staining for Flow Cytometry
Protocol: Cell Surface Antibody Staining for Flow Cytometry
Extracellular Staining for Flow Cytometry There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for ...
Protocol: Intracellular Antibody Staining for Flow Cytometry
Protocol: Intracellular Antibody Staining for Flow Cytometry
Intracellular Staining for Flow Cytometry There are many methods for fixation and permeabilization of cells for flow cytometry. Protocols may ...
Troubleshooting Tips for Fluorescence Staining
Troubleshooting Tips for Fluorescence Staining
Problem: No Staining or Low Signal Primary antibody is not validated for application Check the supplier information to find out if your primary ...
Protocol: Fluorescent Western Blotting
Protocol: Fluorescent Western Blotting
To learn more about western blotting, including the advantages of near-infrared fluorescence detection, see our webinar: Fundamentals of Western ...
Protocol: DNA Probe Labeling by PCR
Protocol: DNA Probe Labeling by PCR
This protocol can be adapted to use dUTP or dCTP labeled with other dyes, biotin, or haptens like digoxigenin. See our full selection of labeled ...
Protocol: Staining Cells with Hoechst or DAPI Nuclear Stains
Protocol: Staining Cells with Hoechst or DAPI Nuclear Stains
Jump to a section: Introduction Protocol for staining live cells Protocol for staining fixed cells or tissue sections Staining bacteria or yeast ...
Protocol: Using Pluronic® F-127 to Solubilize Dyes for Cell Loading
Protocol: Using Pluronic® F-127 to Solubilize Dyes for Cell Loading
Pluronic® F-127 is a nonionic detergent useful for solubilizing hydrophobic molecules in aqueous solutions. Pluronic® F-127 is commonly used to ...
Protocol: Succinimidyl Ester Labeling of Protein Amines
Protocol: Succinimidyl Ester Labeling of Protein Amines
Dyes functionalized with succinimidyl ester (NHS Ester or SE) groups can be used to covalently label proteins on primary amines (typically lysine ...
Protocol: Maleimide Labeling of Protein Thiols
Protocol: Maleimide Labeling of Protein Thiols
Dyes functionalized with a maleimide group can react with thiol groups to form thioether-coupled products. The reaction can take place at neutral ...
Protocol: Aminooxy Labeling of Glycoproteins
Protocol: Aminooxy Labeling of Glycoproteins
Dyes functionalized with an aminooxy reactive group can be used to label molecules with a carbonyl group (aldehyde or ketone) to form a stable ...