Support & Resources

+1

Protocols Library

May 2, 2019By Lori Roberts, PhD
Antibody Staining Protocols Considerations for Immunofluorescence Immunofluorescence Staining ...

Tech Tip: Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits

May 2, 2019By NeuralabAdmin
Introduction Biotium’s Tyramide Amplification Kits are powerful tools to achieve ...

Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence

March 28, 2019By Lori Roberts, PhD
CellBrite™ Cytoplasmic Membrane Dyes are membrane dyes that are useful for visualizing ...

Tech Tip: Five steps to successful cell membrane staining & imaging

October 1, 2018By Lori Roberts, PhD
Tips for success with CellBrite™, CellBrite™ Fix, and MemBrite™ Fix ...

Tech Tip: Cell Surface Stains for Live & Fixed Cells

August 30, 2018By Laurel Mason
Membrane staining is very useful in defining cell boundaries in multicolor imaging studies. ...

Tech Tip: Eight things to keep in mind for optimal gel staining with GelRed® and GelGreen®

July 6, 2018By Laurel Mason
The most common use of GelRed® and GelGreen® nucleic acid stains is in pre-cast agarose gels, ...

Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture

May 31, 2018By Lori Roberts, PhD
ViaFluor®  SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells ...

Tech Tip: Imaging Bacteria Using Agarose Pads

September 14, 2017By Alexis Madrid, PhD
Tech tip: Imaging bacteria using the agarose pad technique Bacteria can be more difficult to ...

Tech Tip: Battling Tissue Autofluorescence

August 11, 2017By NeuralabAdmin
Immunofluorescence staining offers a variety of advantages compared to colorimetric ...

Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst

August 11, 2017By NeuralabAdmin
Cross-talk and specificity in multi-color imaging experiments Cross-talk between fluorescence ...

Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

August 11, 2017By NeuralabAdmin
Immunofluorescence staining (IF) can be performed in two ways, by direct or indirect detection. ...

Protocol: Immunofluorescence Staining of Cells for Microscopy

August 10, 2017By Lori Roberts, PhD
There are many variations on IF protocols, and steps may need to be optimized for different ...

Protocol: Cell Surface Antibody Staining for Flow Cytometry

August 9, 2017By Lori Roberts, PhD
Extracellular Staining for Flow Cytometry There are many protocols for staining cells for flow ...

Protocol: Intracellular Antibody Staining for Flow Cytometry

August 8, 2017By Lori Roberts, PhD
Intracellular Staining for Flow Cytometry There are many methods for fixation and ...

Troubleshooting Tips for Fluorescence Staining

August 7, 2017By Lori Roberts, PhD
Problem: No Staining or Low Signal Primary antibody is not validated for application Check the ...

Protocol: Fluorescent Western Blotting

August 6, 2017By Lori Roberts, PhD
To learn more about western blotting, including the advantages of near-infrared fluorescence ...

Protocol: DNA Probe Labeling by PCR

August 5, 2017By Lori Roberts, PhD
This protocol can be adapted to use dUTP or dCTP labeled with other dyes, biotin, or haptens ...

Protocol: Staining Cells with Hoechst or DAPI Nuclear Stains

August 4, 2017By Lori Roberts, PhD
Jump to a section: Introduction Protocol for staining live cells Protocol for staining fixed ...

Protocol: Succinimidyl Ester Labeling of Protein Amines

August 3, 2017By Lori Roberts, PhD
Dyes functionalized with succinimidyl ester (NHS Ester or SE) groups can be used to covalently ...

Protocol: Maleimide Labeling of Protein Thiols

August 2, 2017By Lori Roberts, PhD
Dyes functionalized with a maleimide group can react with thiol groups to form ...