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MIX-N-STAIN CF™ DYE ANTIBODY LABELING KITS

Simplified Antibody Labeling
Revolutionary technology that dramatically simplifies the process of antibody labeling
Virtually any amount of BSA
Antibody labeling can be conducted in the presence of virtually any amount of BSA, gelatin or non-antibody proteins in ascites fluid when the new, modified protocol is used.
Superior CF™ dyes
More photostable than Alexa Fluor® dyes

Mix-n-Stain™ antibody labeling kits are a revolutionary technology that dramatically simplifies the process of antibody labeling. The kits enable a researcher to covalently label ≤5 ug up to 100 ug of an antibody with one of 20 super bright and photostable CF™ dyes, biotin, or FITC in only 30 minutes and 100% recovery of antibody after labeling (Figure 1). The prepared conjugates perform comparably to  pre-labeled antibodies from leading suppliers. Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging (Figure 2).

Each Mix-n-Stain™ kit comes with everything you need to perform the conjugation. Simply mix your antibody with a vial of pre-measured CF™ dye, biotin, or FITC in the buffer provided, a step taking less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.

mix n stain antibody labeling

30 minute incubation & 100% recovery

CF488A GAM min x rat, CF555 Mix-n-Stain, CF640R phalloidin-IHC

Figure 2. Frozen section of rat testis stained with Mix-n-Stain CF488A labeled mouse anti-tubulin (microtubules, green), Mix-n-Stain CF555 labeled mouse anti-ZO1 (tight junctions, red), and CF640R phalloidin (cat. no. 00050) (actin filaments, cyan).

One breakthrough feature of Mix-n-Stain™ kits is that antibody labeling can be conducted in the presence of virtually any amount of BSA, gelatin or non-antibody proteins in ascites fluid when the new, modified protocol is used. Azide and low concentrations of Tris are also tolerated; an ultrafiltration spin column is included in the kit to quickly and easily remove incompatible small molecules like glycerol, glycine, or high concentrations of Tris. As a result, the kits are compatible with most commercial antibody formulations without an antibody purification step prior to the conjugation process.

Another major advantage of the labeling kits is our superior CF™ dyes, many of which are brighter and more photostable than Alexa Fluor® dyes and other similar next-generation fluorescent dyes. With a collection of 20 CF™ dyes spanning the entire visible and near-infrared spectra, you have unprecedented freedom to label any primary antibody with a color of your choice or biotin, making multi-color detection simple and affordable.


Frequently Asked Questions and Answers:

Q: Is the labeling chemical or physical?

A: The labeling is chemical, which means the dye is covalently attached to the antibody. Covalent labeling ensures no dye transfer or diffusion in multi-color detection employing multiple antibodies labeled with different CF Mix-n-Stain kits.

Q: What are the advantages of Mix-n-Stain kits over Invitrogen’s Zenon® antibody labeling kits?

A: There are four major advantages: 1) Unlike Zenon, Mix-n-Stain labeling covalently attaches the dye to the antibody, eliminating dye transfer or diffusion between antibodies during multi-color staining; 2) Mix-n-Stain conjugates are stable for at least 6 months in storage buffer, whereas Zenon complexes must be used within 30 minutes; 3) Mix-n-Stain conjugates are less bulky because the dyes are directly linked to the antibody, unlike Zenon complexes which use antibody fragments; 4) No post-staining fixation is required with Mix-n-Stain; 5) Unlike Zenon, Mix-n-Stain labeling is not species-specific.

Mix-n-Stain Biotin Antibody Labeling Kit

From: $100 Sizes: 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labelingCatalog #: 92244, 92266, - 92286View allHide

Mix-n-Stain CF Dye Antibody Labeling Kits

From: $95 Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92333, 92285, 92284, 92283, 92282, 92281, 92280, 92279, 92278, 92277, 92276, 92275, 92274, 92273, 92272, 92271, 92270, 92287, 92288, 92289, 92332, 92331, 92324, 92323, 92322, 92321, 92320, 92319, 92318, 92317, 92316, 92305, 92304, 92303, 92291, 92290, 92270, 92268, 92267, 92246, 92245, 92243, 92242, 92241, 92240, 92239, 92238, 92237, 92236, 92235, 92234, 92233, 92232, 92231, 92230, 92247, 92248, 92250, 92265, 92264, 92263, 92262, 92261, 92260, 92259, 92258, 92257, 92256, 92255, 92254, 92253, 92252, 92251, 92250, - 92230View allHide

Mix-n-Stain CF Dye Small Ligand Labeling Kits

From: $158 Sizes: 1 labelingCatalog #: 92350, 92363, 92362, 92361, 92360, 92359, 92358, 92357, 92356, 92355, 92354, 92353, 92352, 92351, - 92364View allHide

Faq

Mix-n-Stain™ Antibody Labeling Kits

CF dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF dyes, the CF Dye Selection Guide, and our CF Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

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See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines. You can download CF™ dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs
See the Mix-n-Stain Kit Selection Guide, below:
Antibody formulationKit and protocol selectionNotes/Examples
Purified IgG containing:

• Sodium azide

• Less than 20 mM Tris

• Less than 10% glycerol
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• More than 20 mM Tris

• More than 10% glycerol

• Glycine

• Less than 0.5 ug/uL IgG
1. Choose the kit size based on the amount of IgG you wish to label

2. Perform ultrafiltration using the spin vial provided in the kit

3. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• Less than 4:1 BSA:IgG by weight

• Less than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS contain­ing 0.1% BSA. The BSA:IgG ratio by weight is 5 ug BSA:5 ug IgG or 1:1. Select a 5-20 ug-sized kit and follow the standard protocol.
Purified IgG containing:

• More than 4:1 BSA:IgG by weight

• More than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the total amount of protein (IgG + BSA/gelatin) in the volume of antibody solution you wish to label

2. Use the modified labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS containing 1% BSA. The BSA:IgG ratio by weight is 50 ug BSA: 5 ug IgG or 10/1. Select a 50-100 ug-sized kit based on 55 ug of total protein in the labeling reaction and follow the modified labeling protocol. If the total protein amount falls between two kit sizes, you may get better results with the larger kit size.
IgG in ascites fluid1. Determine the concentration of protein in the ascites fluid

2. Choose the kit size based on the total amount of protein in the volume of ascites fluid you wish to label

3. Use the modified labeling protocol
For example, if you wish to label 10 uL ascites fluid containing 70 ug total protein. Select a 50-100 ug-sized kit based on 70 ug of total protein, and follow the modified labeling protocol.
Less than 5 ug purified IgG1. Add stabilizer protein such as BSA to the antibody to bring the total amount of protein 5 ug

2. Select a 5-20 ug kit and use the standard labeling protocol
For example, if you wish to label 1 ug IgG sample. Select a 5-20 ug-sized kit. Before labeling, add 4 ug BSA to 1 ug IgG and follow the standard labeling protocol.
IgG in:

• Serum

• Hybridoma cell culture supernatant
Not compatible due to the low concentration of IgG compared to other proteins in these formats; purify IgG before labelingUse protein A/G or a commercially available IgG clean-up kit to purify IgG. Determine the concentration of IgG, then select the appropriate kit/protocol based on the amount of IgG you wish to label and the buffer formulation after purification.

Category: Mix-n-Stain™ Antibody Labeling Kits

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