Calcium Indicators & Chelators

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

TPEN (Tetrakis-(2-pyridylmethyl)ethylenediamine) is an excellent heavy metal chelator while it does not affect calcium, magnesium, sodium, or potassium concentrations. Thus, TPEN is a useful tool to identify effects of heavy metals (zinc, iron, copper, manganese, etc.) on fluorescent indicators for other ions.

DMNP-EDTA (also known as DM-Nitrophen™) is a caged calcium chelator. Upon photolysis, the Kd for Ca²⁺ increases from 5 nM to 3 mM, resulting in a pulse of free Ca²⁺.

Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading.

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation.

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K_d of 1.9 mM (1,2).

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K_d of 1.9 mM.

Fura-2, pentasodium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.

Fura-2, pentapotassium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Fura-2, pentaammonium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.

Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.