Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents
We offer a wide spectrum of CF® Dye colors with a full selection of reactive chemistries, as well as other high quality reactive dyes and biotinylation reagents. This category also includes non-reactive free acid versions of the dyes. Reactive chemistries include:
- Succinimidyl esters (SE, also known as NHS esters) which react with primary amines such as lysine residues in proteins.
- Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) which react with aldehydes or ketones to form a stable oxime bond.
- Maleimides which react with thiol groups to form thioether-coupled products.
- Dyes with an amine group can be used to conjugate the dye to carboxylic acids groups in biological molecules if the carboxylic acids have been activated using water soluble carbodiimides like EDC.
Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide
33000, 33003, 33006, 33009, 33012, 33015, - 33018View allHide
Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.
Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye or Biotin Tyramide
33001, 33004, 33007, 33010, 33013, 33016, - 33019View allHide
Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.
Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide
33002, 33005, 33008, 33011, 33014, 33017, - 33020View allHide
Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.
CF® Dye & Biotin SE Protein Labeling Kits
92208, 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide
Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.
CF® Dye Alkyne
92086, 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide
CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.
CF® Dye Amine
92035, 92036, 92037, 92038, 92039, 92040, 92041, 92042, 92043, 92065, 92102, - 96010View allHide
CF® Dyes with a reactive amine group to fluorescently label carboxylic acid groups in proteins and other molecules.
CF® Dye Aminooxy
92050, 92051, 92052, 92053, 92054, 92055, 92056, 92057, 92058, - 92059View allHide
CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.
CF® Dye Azide
92080, 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
CF® Dye BCN
96070, 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.
CF® Dye Hydrazide
96063, 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 96024, - 96025View allHide
Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
CF® Dye Methyltetrazine
96028, 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide
CF® Dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.
CF® Dye MTS
92096, 92097, 92098, - 92099View allHide
CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.
CF® Dye Picolyl Azide
92187, 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide
CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.
CF® Dye SE/TFP Ester
96109, 97502, 97500, 96095, 96094, 96084, 96073, 96078, 96068, 96067, 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 96092, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide
CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.
CF® Dye TCO
96040, 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide
CF® Dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.
CF® Dye Tetrazine
96055, 96054, 96056, 96096, - 96097View allHide
CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.