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Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

We offer a wide spectrum of CF® dye colors with a full selection of reactive chemistries, as well as other high quality reactive dyes and biotinylation reagents. This category also includes non-reactive free acid versions of the dyes. Reactive chemistries include:

  • Succinimidyl esters (SE, also known as NHS esters) which react with primary amines such as lysine residues in proteins.
  • Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) which react with aldehydes or ketones to form a stable oxime bond.
  • Maleimides which react with thiol groups to form thioether-coupled products.
  • Dyes with an amine group can be used to conjugate the dye to carboxylic acids groups in biological molecules if the carboxylic acids have been activated using water soluble carbodiimides like EDC.

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    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33000, 33003, 33006, 33009, 33012, 33015, - 33018View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

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    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33001, 33004, 33007, 33010, 33013, 33016, - 33019View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

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    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide

    Sizes: 1 kitCatalog #: 33002, 33005, 33008, 33011, 33014, 33017, - 33020View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

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    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     

    CF® Dye & Biotin SE Protein Labeling Kits

    From: $394 Sizes: 3 labelingsCatalog #: 92208, 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide

    Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

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    Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

     

    CF® Dye Alkyne

    Sizes: 0.5 mgCatalog #: 92086, 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide

    CF® dye alkynes react with azide groups.  Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

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    CF® dye alkynes react with azide groups.  Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

     

    CF® Dye Amine

    Sizes: 1 mgCatalog #: 92035, 92036, 92037, 92038, 92039, 92040, 92041, 92042, 92043, 92065, 92102, - 96010View allHide

    CF® dyes with a reactive amine group to fluorescently label carboxylic acid groups in proteins and other molecules.

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    CF® dyes with a reactive amine group to fluorescently label carboxylic acid groups in proteins and other molecules.

     

    CF® Dye Aminooxy

    Sizes: 1 mgCatalog #: 92050, 92051, 92052, 92053, 92054, 92055, 92056, 92057, 92058, - 92059View allHide

    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

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    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

     

    CF® Dye Azide

    Sizes: 0.5 mgCatalog #: 92080, 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide

    CF® dyes with an azide group. Can be used to label and detect alkyne, BCN or cyclooctyne-containing molecules following azide-alkyne cycloaddition by fluorescence spectroscopy, microscopy or flow cytometry .

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    CF® dyes with an azide group. Can be used to label and detect alkyne, BCN or cyclooctyne-containing molecules following azide-alkyne cycloaddition by fluorescence spectroscopy, microscopy or flow cytometry .

     

    CF® Dye BCN

    Sizes: 0.5 mgCatalog #: 96070, 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide

    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

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    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

     

    CF® Dye Hydrazide

    Sizes: 1 mgCatalog #: 96063, 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 92192, 96024, - 96025View allHide

    Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

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    Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

     

    CF® Dye Methyltetrazine

    Sizes: 1 mgCatalog #: 96028, 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide

    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

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    CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

     

    CF® Dye MTS

    Sizes: 1 mgCatalog #: 92096, 92097, 92098, - 92099View allHide

    MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.

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    MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.

     

    CF® Dye Picolyl Azide

    Sizes: 0.5 mgCatalog #: 92187, 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

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    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

     

    CF® Dye Succinimidyl Ester

    From: $151 Sizes: 0.25 umol, 1 umolCatalog #: 96073, 96078, 96068, 96067, 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide

    CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

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    CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

     

    CF® Dye TCO

    Sizes: 1 mgCatalog #: 96040, 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide

    CF dye with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

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    CF dye with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

     

    CF® Dye Tetrazine

    Sizes: 1 mgCatalog #: 96056, 96055, - 96054View allHide

    CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

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    CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.