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Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

We offer a wide spectrum of CF® Dye colors with a full selection of reactive chemistries, as well as other high quality reactive dyes and biotinylation reagents. This category also includes non-reactive free acid versions of the dyes. Reactive chemistries include:

  • Succinimidyl esters (SE, also known as NHS esters) which react with primary amines such as lysine residues in proteins.
  • Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) which react with aldehydes or ketones to form a stable oxime bond.
  • Maleimides which react with thiol groups to form thioether-coupled products.
  • Dyes with an amine group can be used to conjugate the dye to carboxylic acids groups in biological molecules if the carboxylic acids have been activated using water soluble carbodiimides like EDC.

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    Product

    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide

    From: $564 Sizes: 1 kitCatalog #:
    33000
    , 33003, 33006, 33009, 33012, 33015, - 33018View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     
    Product

    Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF® Dye or Biotin Tyramide

    From: $564 Sizes: 1 kitCatalog #:
    33001
    , 33004, 33007, 33010, 33013, 33016, - 33019View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     
    Product

    Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide

    From: $564 Sizes: 1 kitCatalog #:
    33002
    , 33005, 33008, 33011, 33014, 33017, - 33020View allHide

    Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

     
    Product

    CF® Dye & Biotin SE Protein Labeling Kits

    From: $480 Sizes: 3 labelingsCatalog #:
    92208
    , 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, 92217, 92218, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, - 92228View allHide

    Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

     
    Product

    CF® Dye Alkyne

    From: $329 Sizes: 0.5 mgCatalog #:
    92086
    , 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide

    CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

     
    Product

    CF® Dye Amine

    From: $251 Sizes: 1 mgCatalog #:
    92035
    , 92036, 92037, 92038, 92039, 92040, 92041, 92042, 92043, 92065, 92102, - 96010View allHide

    CF® Dyes with a reactive amine group to fluorescently label carboxylic acid groups in proteins and other molecules.

     
    Product

    CF® Dye Aminooxy

    From: $513 Sizes: 1 mgCatalog #:
    92050
    , 92051, 92052, 92053, 92054, 92055, 92056, 92057, 92058, - 92059View allHide

    CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.

     
    Product

    CF® Dye Azide

    From: $329 Sizes: 0.5 mgCatalog #:
    92080
    , 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide

    CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

     
    Product

    CF® Dye BCN

    From: $389 Sizes: 0.5 mgCatalog #:
    96070
    , 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide

    CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

     
    Product

    CF® Dye Hydrazide

    From: $199 Sizes: 1 mgCatalog #:
    96063
    , 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 96024, - 96025View allHide

    Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

     
    Product

    CF® Dye Methyltetrazine

    From: $343 Sizes: 1 mgCatalog #:
    96028
    , 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide

    CF® Dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

     
    Product

    CF® Dye MTS

    From: $209 Sizes: 1 mgCatalog #:
    92096
    , 92097, 92098, - 92099View allHide

    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

     
    Product

    CF® Dye Picolyl Azide

    From: $340 Sizes: 0.5 mgCatalog #:
    92187
    , 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

     
    Product

    CF® Dye SE/TFP Ester

    From: $184 Sizes: 5 mg, 0.25 umol, 1 umolCatalog #:
    97502
    , 97500, 96095, 96094, 96084, 96073, 96078, 96068, 96067, 92137, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 96092, 92133, 92134, 92135, 92155, 92103, 92104, 92105, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, - 92120View allHide

    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

     
    Product

    CF® Dye TCO

    From: $343 Sizes: 1 mgCatalog #:
    96040
    , 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide

    CF® Dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.

     
    Product

    CF® Dye Tetrazine

    From: $334 Sizes: 1 mgCatalog #:
    96055
    , 96054, 96056, 96096, - 96097View allHide

    CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.