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Yeast Stains

Biotium offers a variety of dyes and stains that have been validated for use in yeast.

Organelle stains: MitoView™ dyes to stain mitochondria, LysoView™ dyes to stain the vacuole, Thiazole Orange to stain the nucleus.

Cell wall stains: Calcofluor White and Concanavalin A (ConA). Wheat Germ Agglutinin (WGA) localizes to bud scars.

Viability and metabolism: Live-or-Dye™ Fixable Live/Dead Kit, Viability Staining Kit with 3 color combos to choose from, and Vitality Staining Kit, containing the metabolic reporter ViaVac™ Red/Green (also available as a standalone dye).

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    MitoView™ Dyes

    From: $40 Sizes: Trial size (50 ug), 20 x 50 ugCatalog #: 70070, 70054, 70055, 70070-T, 70054-T, 70055-T, 70068, - 70068-TView allHide

    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

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    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

     

    ViaFluor® SE Cell Proliferation Kits

    From: $50 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #: 30050, 30068, 30086, 30068-T, - 30086-TView allHide

    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

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    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

     

    LysoView™ Dyes

    From: $40 Sizes: Trial size (10 uL), 50 uL, Trial size (1 vial), Set of 10 vialsCatalog #: 70058, 70061, 70061-T, 70058-T, 70059-T, 70059, 70067, 70067-T, 70066, - 70066-TView allHide

    LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. Far-red LysoView™ 650 is compatible with super-resolution imaging by STED and SIM. Note: LysoView™650 (70059 and 70059-T) is temporarily unavailable.

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    LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. Far-red LysoView™ 650 is compatible with super-resolution imaging by STED and SIM. Note: LysoView™650 (70059 and 70059-T) is temporarily unavailable.

     

    ViaVac™ Red/Green

    From: $190 Sizes: 100 uLCatalog #: 29068

    ViaVac™ Red/Green is a fluorescent viability dye for yeast, identical in structure to the dye FUN® 1. ViaVac passively diffuses into cells, showing green cytoplasmic staining.

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    ViaVac™ Red/Green is a fluorescent viability dye for yeast, identical in structure to the dye FUN® 1. ViaVac passively diffuses into cells, showing green cytoplasmic staining.

     

    Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit

    From: $350 Sizes: 1,000 assaysCatalog #: 31064

    The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

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    The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

     

    Yeast Viability Staining Kit

    From: $380 Sizes: 1,000 assaysCatalog #: 31063-1, 31063-2, - 31063-3View allHide

    The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components, CF® Dye-labeled Concanavalin A (ConA) and Live-or-Dye® viability dye.

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    The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components, CF® Dye-labeled Concanavalin A (ConA) and Live-or-Dye® viability dye.

     

    Yeast Vitality Staining Kit

    From: $210 Sizes: 1,000 assaysCatalog #: 31062

    The Yeast Vitality Staining Kit is a fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. The kit contains two fluorescent components, Calcofluor White and ViaVac Red/Green.

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    The Yeast Vitality Staining Kit is a fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. The kit contains two fluorescent components, Calcofluor White and ViaVac Red/Green.

     

    Calcofluor White Stain, 5 mM in Water

    From: $53 Sizes: 20 mLCatalog #: 29067

    Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin, which can be found in the cell walls of fungi, algae, and plants. In budding yeast Calcofluor White stains bud scars more strongly, because they have a higher concentration of chitin.

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    Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin, which can be found in the cell walls of fungi, algae, and plants. In budding yeast Calcofluor White stains bud scars more strongly, because they have a higher concentration of chitin.

     

    Concanavalin A (Con A) CF® Dye Conjugates

    From: $144 Sizes: 5 x 1 mgCatalog #: 29015, 29016, 29017, 29018, 29019, 29020, 29058, 29074, 29075, - 29080View allHide

    This product is concanavalin A (Con A) labeled with our superior fluorescent CF® dyes. Con A is a widely used lectin that selectively binds to a-mannopyranosyl and a-glucopyranosy residues, found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells.

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    This product is concanavalin A (Con A) labeled with our superior fluorescent CF® dyes. Con A is a widely used lectin that selectively binds to a-mannopyranosyl and a-glucopyranosy residues, found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells.

     

    Light-on LysoView™ 555

    From: $40 Sizes: 10 uL, 50 uLCatalog #: 70060, - 70060-TView allHide

    Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is activated by exposure to UV excitation.

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    Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is activated by exposure to UV excitation.

     

    Thiazole Orange, 10 mM in DMSO

    From: $85 Sizes: 1 mLCatalog #: 40077

    Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.

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    Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange also functions as a fixable live-cell cytoplasmic stain in yeast cells. Supplied at 10 mM in DMSO.

     

    Wheat Germ Agglutinin (WGA) Conjugates

    From: $66 Sizes: 1 mg, 5 x 1 mgCatalog #: 29021, 29022, 29023, 29024, 29025, 29026, 29027, 29028, 29029, 29059, 29064, 29073, 29021-1, 29022-1, 29023-1, 29024-1, 29025-1, 29026-1, 29027-1, 29028-1, 29029-1, 29059-1, 29064-1, 29077-1, 29077, 29076-1, - 29076View allHide

    This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.

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    This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.