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Membrane Potential & Nerve Terminal Dyes

Biotium offers a wide selection of synaptic vesicle dyes, as well as fast-responding and slow-responding membrane potential dyes. Learn more about Probes for Neuroscience Research and Ion Indicators & Membrane Potential Dyes.

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    DiO/DPA Membrane Potential Detection Kit

    From: $205 Sizes: 1 kitCatalog #: 30037

    The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a “stationary” FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change.

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    The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a “stationary” FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change.

     

    Nerve Terminal Staining Kits

    From: $226 Sizes: 1 setCatalog #: 70030, 70031, 70032, 70034, - 70031-1View allHide

    Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence.

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    Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and accessories for washing away or quenching extracellular dye fluorescence.

     

    SynaptoGreen™ C1

    From: $206 Sizes: 5 mg, 5 x 1 mgCatalog #: 70042, - 70043View allHide

    SynaptoGreen™ C1 has the shortest (one-carbon) lipophilic tail and is the most water soluble of the nerve terminal dye series. Thus it is expected to have the slowest on-rate and the fastest off-rate among the SynaptoGreen™ dyes.

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    SynaptoGreen™ C1 has the shortest (one-carbon) lipophilic tail and is the most water soluble of the nerve terminal dye series. Thus it is expected to have the slowest on-rate and the fastest off-rate among the SynaptoGreen™ dyes.

     

    SynaptoGreen™ C18 (equivalent to FM®3-25)

    From: $316 Sizes: 5 mg, 5 x 1 mgCatalog #: 70048, - 70049View allHide

    SynaptoGreen™ C18 (equivalent to FM®3-25) is a membrane stain similar to their nerve terminal dyes, except that it has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.

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    SynaptoGreen™ C18 (equivalent to FM®3-25) is a membrane stain similar to their nerve terminal dyes, except that it has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.

     

    SynaptoGreen™ C2 (Equivalent to FM®2-10)

    From: $206 Sizes: 5 mg, 5 x 1 mgCatalog #: 70044, - 70045View allHide

    SynaptoGreen™ C2 is more water soluble than SynaptoGreen™ C4 and thus has a faster de-staining rate than the latter.

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    SynaptoGreen™ C2 is more water soluble than SynaptoGreen™ C4 and thus has a faster de-staining rate than the latter.

     

    SynaptoGreen™ C3

    From: $206 Sizes: 5 mg, 5 x 1 mgCatalog #: 70023, - 70026View allHide

    SynaptoGreen™ C3 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon shorter and also the hydrophilic end is a trimethylammonium group.

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    SynaptoGreen™ C3 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon shorter and also the hydrophilic end is a trimethylammonium group.

     

    SynaptoGreen™ C4 (Equivalent to FM®1-43)

    From: $267 Sizes: 5 mg, 5 x 1 mgCatalog #: 70020, - 70022View allHide

    A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.

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    A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.

     

    SynaptoGreen™ C5 (Equivalent to FM®1-84)

    From: $206 Sizes: 5 mg, 5 x 1 mgCatalog #: 70046, - 70047View allHide

    SynaptoGreen™ C5 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon longer.

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    SynaptoGreen™ C5 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon longer.

     

    SynaptoRed™ C2 (equivalent to FM®4-64)

    From: $267 Sizes: 5 mg, 5 x 1 mgCatalog #: 70021, - 70027View allHide

    A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

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    A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

     

    SynaptoRed™ C2M (equivalent to FM®5-95)

    From: $267 Sizes: 5 mg, 5 x 1 mgCatalog #: 70019, - 70028View allHide

    SynaptoRed™ C2M is a red fluorescent probe used to follow synaptic vesicles.

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    SynaptoRed™ C2M is a red fluorescent probe used to follow synaptic vesicles.

     

    SynaptoRed™C1

    From: $206 Sizes: 5 mg, 5 x 1 mgCatalog #: 70040, - 70041View allHide

    SynaptoRed™ C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed™ C2 (also called FM4-64) except that the lipophilic tail of SynaptoRed™ C1 is one carbon shorter than that of the SynaptoRed™ C2.

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    SynaptoRed™ C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed™ C2 (also called FM4-64) except that the lipophilic tail of SynaptoRed™ C1 is one carbon shorter than that of the SynaptoRed™ C2.

     

    ADVASEP-7

    From: $204 Sizes: 250 mgCatalog #: 70029

    ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (also called FM1-43, catalog no. 70020) to stain brain slices.

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    ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (also called FM1-43, catalog no. 70020) to stain brain slices.

     

    AM1-43

    From: $193 Sizes: 1 mgCatalog #: 70024

    AM1-43 is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.

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    AM1-43 is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.

     

    AM1-44

    From: $213 Sizes: 1 mgCatalog #: 70038

    AM1-44 is a version of AM1-43 (catalog no. 70024) with improved fixability. The dye can also be used as a general probe for following endocytosis.

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    AM1-44 is a version of AM1-43 (catalog no. 70024) with improved fixability. The dye can also be used as a general probe for following endocytosis.

     

    AM2-10

    From: $193 Sizes: 1 mgCatalog #: 70036

    AM2-10 is a fixable analog of SynaptoGreen™ C2.

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    AM2-10 is a fixable analog of SynaptoGreen™ C2.

     

    AM3-25

    From: $256 Sizes: 1 mgCatalog #: 70051

    AM3-25 is a lipophilic styryl dye that has an amine group at the hydrophilic head. The amine group reacts with formaldehyde or glutaldehyde to make the dye fixable.

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    AM3-25 is a lipophilic styryl dye that has an amine group at the hydrophilic head. The amine group reacts with formaldehyde or glutaldehyde to make the dye fixable.