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FLOW CYTOMETRY REAGENTS

Rapid and convenient antibody labeling kits and over 1000 monoclonal antibodies
Bright, fixable dead cell stains, available in 14 colors with options for spectral flow
Non-toxic, fixable proliferation dyes designed as improvements to CFSE
Novel and classic stains for analyzing DNA content/cell cycle by flow cytometry
Monitor apoptosis with the worlds only live-cell, real-time caspase-3 substrates
Dyes for monitoring mitochondrial membrane potential and apoptosis/necrosis status

Simplify your panel design with CF® Dyes

Our industry-leading CF® dyes boast superior signal-to noise and offer the widest selection of spectrally unique dyes to accommodate highly complex panels.

CF® Dye Advantages

  • Available in over 30 colors, offering the greatest coverage across visible and near-IR wavelengths
  • Widest selection of spectrally unique dyes to accomodate highly complex panels
  • CF® Dye antibody conjugates have superior brightness and signal-to-noise over alternative fluorophores

Learn more about how CF® dyes offer advantages for enhanced multiplexing in spectral flow cytometry.

CF® dyes are available in more than 30 bright fluorescent colors from blue to near-IR.

PRIMARY ANTIBODIES & ANTIBODY LABELING KITS

Primary Antibodies

  • Growing collection of more than 1000 monoclonal antibodies: View Flyer
  • Choose from 13 bright CF® dyes, R-PE, APC, PerCP, HRP, AP, or biotin
  • Isotype control antibodies available conjugated to the same dyes and labels
  • Available BSA-free, ready to use for Mix-n-Stain™ labeling
  • Affordable 100 uL sizes available

Browse primary antibodies, or search with Antibody Finder

Primary Antibodies – sizes and formats:

FormatConcentrationSize
CF® dye conjugates (13 colors)0.1 mg/mL100 uL or 500 uL
Biotin, HRP, or AP conjugates0.1 mg/mL100 uL or 500 uL
R-PE, APC, or Per-CP conjugates0.1 mg/mL250 uL
Purified, with BSA0.2 mg/mL100 uL or 500 uL
Purified, BSA-free (Mix-n-Stain™ Ready)1 mg/mL50 uL

 

Streptavidin & Anti-Biotin Secondary Antibodies

Streptavidin and anti-biotin antibodies available in 21 bright CF® dyes, R-PE, APC, and PerCP

View our full product listing of anti-tag secondary antibodies here.

Antibody Labeling Kits

Labeling your own antibodies? Our Mix-n-Stain™ Antibody Labeling Kits dramatically simplify the process of preparing fluorescently-labeled antibodies, offering bright and stable conjugates in only 30 minutes. The resulting conjugates offer comparable performance to antibodies labeled with other commercially available labeling kits (Fig. 1.) Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging and multiplex flow cytometry.

  • Label with your choice of 29 CF® dyes in only 30 minutes
  • Labeling Kits for FITC, Biotin, R-PE, APC and other labels also available
  • Stable covalent labeling with no purification required
  • Compatible with common antibody stabilizers
  • Labeling kits designed for Nanobodies® (ie. camelid single variable or VHH domains) also available
Figure 1. Anti-mouse secondary antibody was labeled with APC using either the Mix-n-Stain™ APC Labeling Kit, or a competitor’s APC labeling kit. The antibodies were used to detect mouse anti-CD3 staining of Jurkat cells. The Mix-n-Stain™ labeled antibody showed lower background without primary antibody (pink) and higher signal on CD3-labeled cells (blue).

Learn more about full selection of Mix-n-Stain™ Labeling Kits here.

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LIVE-OR-DYE™ FIXABLE VIABILITY STAINING KITS

Live-or-Dye™ fixable dead cell stains are cell membrane impermeant amine-reactive dyes. The dyes enter dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins (Fig. 2). Live-or-Dye™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. Biotium currently offers 14 Live-or-Dye™ stains, the widest selection of viability stains spanning the fluorescence spectrum for maximal flexibility in designing multi-color flow panels.

The best fixable dead cell stains for flow cytometry:

  • Largest selection of dye colors for easy panel design
  • Dye options designed specially for spectral flow
  • Exceptionally bright dyes for excellent live/dead separation
  • No loss of brightness after fixation
  • For flow cytometry or fluorescence microscopy

Live-or-Dye fixable viability stains
Figure 2. Principle of Live-or-Dye discrimination of live and dead cells.
Figure 3. Live-or-Dye™ kits are available with 14 bright and stable dye colors for multichannel flow cytometry.

Spectral flow cytometry is a rapidly growing technology with significantly enhanced multiplexing capabilities over conventional flow cytometry. Where conventional flow cytometer instruments can detect panels with more than a dozen fluorophores, spectral flow cytometers can accommodate panels with upwards of 40 dyes. Biotium offers spectrally unique Live-or-Dye™ stains designed specifically to take advantage of enhanced multiplexing by spectral flow ctyometry.

Live-or-Dye™ Stains for Spectral Flow

Figure 4. Excitation and emission spectra of Live-or-Dye™ stains designed specifically for spectral flow cytometry. Dotted lines represent excitation spectra, while shaded areas represent emission spectra.

New: Live-or-Dye™ Sampler Kits

Live-or-Dye™ stains are available in two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Live-or-Dye™ Sampler Kits table below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.

Live-or-Dye™ Fixable Viability Staining Kits – 250 assays, Live-or-Dye™ Sampler Kit, Standard
Live-or-Dye™ Fixable Viability Staining Kits – 250 assays, Live-or-Dye™ Sampler Kit, Spectral

To learn more about Live-or-Dye™ Fixable Viability Stains, please visit the Live-or-Dye™ technology page.

Cat. No.

Viability Dye

Compatible lasers (nm)

Optimal detection channels

Notes

32002, 32002-TLive-or-Dye™ 350/448355, 375DAPI
32014,32014-TLive-or-Dye™ 375/600355, 375, 405Spectral Scan, BUV615, BV605Developed for spectral cytometry
32003, 32003-TLive-or-Dye™ 405/452405 nmPacific Blue®, BV421, BV450
32009, 32009-TLive-or-Dye™ 405/545405 nmAmCyan, BV510
32004, 32004-TLive-or-Dye™ 488/515488 nmFITCValidated for microscopy
32012, 32012-TLive-or-Dye™ 510/550488, 532Spectral scanDeveloped for spectral cytometry
32005, 32005-TLive-or-Dye™ 568/583488, 532, 561PE, PIValidated for microscopy
32006, 32006-TLive-or-Dye™ 594/614488, 532, 561PI, PE-CF®594, PE-Texas Red®Validated for microscopy
32015, 32015-TLive-or-Dye™ 615/740633-640Spectral scan, APC-Cy®7Developed for spectral cytometry
32007, 32007-TLive-or-Dye™ 640/662633-640APCValidated for microscopy
32013, 32013-TLive-or-Dye™ 665/685633-640Spectral scan, AF700Developed for spectral cytometry
32008, 32008-TLive-or-Dye™ 750/777633-640, 785APC-Cy®7The brightest dye for the APC-Cy®7 channel
32011, 32011-TLive-or-Dye™ 787/808785, 808APC-Cy®7, IR800

Live-or-Dye™ Sampler Kits

Kit Name

Catalog No.

Included dyes: Ex/Em (Cat No.)

Application

Live-or-Dye™ Fixable Viability Sampler Kit, Standard32016• 350/488 (32002A)
• 405/545 (32009A)
• 488/515 (32004A)
• 568/583 (32005A)
• 640/662 (32007A)
Designed for use on most standard flow cytometry laser and filter configurations
Live-or-Dye™ Fixable Viability Sampler Kit, Spectral32017• 350/448 (32002A)
• 375/600 (32014A)
• 510/550 (32012A)
• 615/740 (32015A)
• 664/683 (32013A)
Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores

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VIAFLUOR® CELL PROLIFERATION

Cell Proliferation Monitoring by Flow Cytometry

Cell proliferation dyes diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 3, 4). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining.

ViaFluor® SE Cell Proliferation Kits

CFSE (also known as CFDA-SE or carboxyfluorescein diacetate, succinimidyl ester) has been used for many years to monitor cell proliferation by flow cytometry. While several alternatives have been developed in more recent years, CFSE remains popular and widely used. Biotium continues to offer CFSE under the tradename ViaFluor® CFSE. However, CFSE has several drawbacks including leakage from the cell, cell toxicity, and bleed-through into the PE and PE-TexasRed® channels.

ViaFluor® 405 SE and ViaFluor® 488 SE cell proliferation dyes were developed at Biotium to provide superior cell staining, fixability, and low toxicity. ViaFluor® 405 is excited with the violet laser and detected in the Pacific Blue® channel, and gives great peaks with no toxicity (Fig. 4). It behaves identically to the popular proliferation dye CellTrace™ Violet (Fig. 4). ViaFluor® 488 and ViaFluor® CFSE are both detected in the FITC channel, but ViaFluor® 488 was developed as a less toxic, less leaky and more fixable alternative to the classic dye CFSE (Fig. 5). It also has less bleed-through into other channels such as PE and PE-Texas-Red®.

Figure 4. Jurkat cells were stained with 5 uM cell proliferation dye: ViaFluor® 405 (left panel) or CellTrace™ Violet (right panel). Cells were analyzed in the Pacific Blue channel immediately after staining, and then every day for four days. Both dyes give sharp peaks showing the progressive decrease in signal caused by cell division.
Figure 5. Jurkat cells were stained with 3 uM cell proliferation dye: ViaFluor® 488 (left panel) or ViaFluor® CFSE (right panel). Cells were analyzed in the FITC channel immediately after staining, and then every day for three days. CFSE loses a lot of signal between initial staining and Day 1, limiting the number of cell divisions that can be measured, while ViaFluor® 488 retains dye better and gives nice sharp peaks for each division cycle.

To learn more about ViaFluor® Cell Proliferation Kits, please visit the ViaFluor® technology page.

Cell Division
Tracking Dyes

Catalog No.

Ex/Em (nm)

Flow detection
channel

Features

ViaFluor® 405 Cell Proliferation Kit30068408/452 Pacific Blue®• Track cell division by dye dilution using flow cytometry

• ViaFluor® 488 is a unique, improved green dye to replace CFSE

• ViaFluor® 405 replaces CellTrace™ Violet
ViaFluor® 488 Cell Proliferation Kit30086493/532FITC
ViaFluor® CFSE Cell Proliferation Kit30050495/519FITC

CELL CYCLE ANALYSIS

RedDot™1 Far-Red Nuclear Stain

RedDot™1 is a novel far-red nuclear stain developed at Biotium. RedDot™1
is a live cell stain similar to DRAQ®5 that can be used for cell cycle
distribution analysis (Fig. 6). In addition, RedDot™1 cell cycle analysis does
not require an RNase step unlike the classic nuclear stain Propidium Iodide
(PI). RedDot™1 can also be used as a far-red nuclear counterstain for live
cells in microscopy.

  • Far-red cell membrane-permeant nuclear dye similar to Draq5™.
  • Can be used for DNA content analysis by flow cytometry like Vybrant™ DyeCycle™ Ruby
  • Highly thermostable and photostable, for convenient handling
RedDot™1 Far-Red Nuclear Stain, 200X in Water – 25 uL
Figure 6. RedDot™1 performs comparably to Vybrant™ DyeCycle™ Ruby for DNA content flow cytometry analysis in live cells. Live Jurkat cells were stained with 1X RedDot™1 or Vybrant™ DyeCycle™ Ruby for 30 minutes, then analyzed using a BD LSRII flow cytometer with 633 nm excitation and 710/50 BP filter.

NucSpot® 470 Nuclear Stain

NucSpot® 470 is a cell membrane-impermeant green fluorescent DNA stain for nuclear counterstaining of fixed cells, or selective staining of dead cells.

  • Nuclear-specific green counterstain for fixed cells
  • Selective detection of dead cells by flow cytometry in the FITC channel
  • Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells (Fig. 7)
  • No wash required
NucSpot® 470 Nuclear Stain, 1000X in DMSO – 20 uL
Figure 7. Cell cycle profiling by flow cytometric DNA content analysis in fixed/permeabilized cells using NucSpot® 470. Jurkat cells were fixed with 4% paraformaldehyde for minutes at RT, then stained with 8X NucSpot® 470 in PBS with 0.1% Triton® X-100 for 15 minutes at room temperature. Cells were analyzed on a BD LSRII flow cytometer in the FITC channel on linear scale.

NucSpot® Far-Red Nuclear Stain

NucSpot® Far-Red is an improved alternative to the popular flow cytometry dead cell dye 7-AAD. It has red-shifted fluorescence emission compared to 7-AAD, for less bleed-through fluorescence in the PE-Texas Red® channel.

  • Improved alternative to 7-AAD with less bleed-through fluorescence in the PE-Texas Red® channel
  • Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
  • Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells (Fig. 8)
  • Substitute for 7-AAD in any standard protocol
NucSpot® Far-Red, 1000X in DMSO – 50 uL
Figure 8. Jurkat cells were fixed with ice-cold methanol, then stained with 10X NucSpot® Far-Red in PBS for 15 minutes. Cells were analyzed on a BD LSRII flow cytometer in the APC channel on linear scale.

Dyes for Cell Cycle Analysis

Product

Catalog No.

Color (Ex/Em*)

Features

RedDot™1 Far-Red Nuclear Stain, 200X in Water 40060Far-red
(662/694 nm)
• For short-term live cell staining (≤4 hours)
• Analyze DNA content/cell cycle by flow cytometry
• Useful for cell number normalization for In Cell Western®
• Can be excited at wavelengths between 488 and 647 nm
• Detect in Cy®5 or APC channel
NucSpot® 470 Nuclear Stain, 1000X in DMSO 40083Green
(460/546 nm)
• Nuclear-specific green counterstain for fixed cells
• Analyze DNA content/cell cycle by flow cytometry
• Selectively stains dead cells in live cultures
• Excellent match for blue LED excitation sources
NucSpot® Far-Red, 1000X in DMSO40085Far-red
(597/667 nm)
• Designed as improved replacement for 7-AAD
• For flow cytometry in the PE-Cy®5 or APC channel
• Less bleed into the PE-Texas Red® channel
Propidium Iodide, 100 mg40016Red
(530/622 nm)
• Widely used dead cell stain
• Can be excited by 488 nm laser line for detection in the PE channel by flow cytometry
• Useful for cell cycle analysis in fixed cells (with RNase treatment)
Propidium Iodide, 1 mg/mL in Water40017
Propidium Iodide Buffer, 50 ug/mL40048
7-AAD, 1 mg 40037Far-red
(546/647 nm)
• Far-red dye for flow cytometry detection in the PE-Cy®5 channel
• Can be excited by the 488 nm or 532 nm laser line
• Useful for cell cycle analysis in fixed cells
7-AAD, 1 mg/mL solution 40084
* When bound to nucleic acids
Cy dye is a registered trademark of GE Healthcare. In Cell Western is a registered trademarks of LI-COR Inc.

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NUCVIEW® CASPASE-3 SUBSTRATES

NucView® Caspase-3 Substrates for live cell apoptosis monitoring

NucView® Caspase-3 Substrates are novel fluorogenic substrates developed by Biotium to detect apoptosis in intact cells in real-time. Biotium has invented a new concept in the design of fluorogenic enzyme substrates. The enzyme substrate is attached to a fluorogenic DNA dye. Before cleavage, the dye is non-fluorescent and unable to bind to DNA. Upon enzymatic cleavage of the substrate, the dye is released and becomes capable of binding to DNA to emit fluorescence. In the case of NucView® Caspase-3 Substrates, the caspase-3/7 substrate peptide DEVD is attached to a DNA-binding dye. The substrate enters the cell cytoplasm where it is cleaved by caspase-3 in apoptotic cells to release the fluorogenic DNA dye, which stains the nucleus (Fig. 9). NucView® can be fixed with formaldehyde after staining, but it cannot be used to stain fixed cells or tissues.

NucView® Advantages

  • Simple, homogenous assay for endpoint assay or real-time detection in live cells
  • Non-toxic, allowing for multi-day experiments
  • Detect caspase-3 activity and visualize apopototic nuclear morphology
  • For flow cytometry, fluorescence microscopy, or live cell imaging systems
  • Available as stand-alone substrates or in kits with other apoptosis or necrosis probes
  • Available with blue, green, or red fluorescence
  • NucView® 488 validated in more than 100 cell types and 200 publications – See the list

Figure 9. Principle of NucView® substrate technology. The substrate is non-fluorescent and does not bind DNA. After enzyme cleavage, the high-affinity DNA dye is released to bind DNA and become fluorescent.
Figure 10. NucView® 405 Caspase-3 Substrate fluorescence over time in staurosporine-treated Jurkat cells, analyzed in the Pacific Blue® channel of a BD LSRII flow cytometer.

To learn more about NucView® Caspase-3 substrates, and kits please visit the NucView® technology page.

NucView® Caspase-3 Substrates and Kits

Catalog No.

Features

NucView® 405 Caspase-3 Substrate, 1 mM in DMSO10405Blue fluorescence for flow cytometry in the Pacific Blue® channel or microscopy with the 405 nm laser
NucView® 405 Caspase-3 Substrate, 1 mM in PBS10407NucView® 405 substrate in PBS, for DMSO-sensitive cell types
NucView® 488 Caspase-3 Substrate, 1 mM in DMSO10402Green fluorescent substrate validated in more than 100 cell types and 200 publications
NucView® 488 Caspase-3 Substrate, 1 mM in PBS10403NucView® 488 substrate in PBS, for DMSO-sensitive cell types
NucView® 530 Caspase-3 Substrate, 1 mM in DMSO10406Orange fluorescence for microscopy in the Cy®3 channel or flow cytometry in the R-PE channel
NucView® 530 Caspase-3 Substrate, 1 mM in PBS10408NucView® 530 substrate in PBS, for DMSO-sensitive cell types
NucView® 488 and MitoView™ 633 Apotosis Detection Kit30062NucView® 488 and far-red fluorescent MitoView™ 633 for the Cy®5 channel
NucView® 488 and RedDot™ 2 Apoptosis & Necrosis Kit30072NucView® 488 and far-red dead cell DNA dye RedDot™ 2 for the Cy®5 channel
Dual Apoptosis Assay with NucView® 488 and CF®594 Annexin V30067NucView® 488 and red fluorescent Annexin V apoptosis probe
Dual Apoptosis Assay with NucView® 488 and CF®640R Annexin V30073NucView® 488 and far-red fluorescent Annexin V apoptosis probe

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CELL VIABILITY ASSAYS

Mitochondrial Membrane Potential

Loss of mitochondrial membrane potential is a hallmark for apoptosis. Biotium offers MitoView™ 633 dye for membrane potential-sensitive staining of mitochondria by microscopy or flow cytometry. For simultaneous detection of mitochondrial membrane potential and caspase-3 activity, see the NucView®488 and MitoView™633 Apoptosis Assay (Fig. 11).

We also offer MitoView™ 405, which changes localization upon mitochondrial depolarization, and MitoView™ Green, a membrane-potential independent mitochondrial dye that can be used to image mitochondria following mitochondrial depolarization, or after fixation.

In healthy cells, JC-1 dye aggregates in mitochondria as a function of membrane potential, resulting in red fluorescence with brightness proportional to the membrane potential. Conversely, in apoptotic and necrotic cells with diminished mitochondrial membrane potential, JC-1 exists in a green fluorescent monomeric form in the cytosol, allowing of cell viability to be assessed by measuring the ratio of red to green fluorescence by flow cytometry or fluorescence microplate reader. Rhodamine 123 is a green fluorescent mitochondrial membrane potential dye. Red fluorescent TMRM and TMRE are the preferred dyes for quantitative membrane potential measurements.


Figure 11. Timecourse of NucView®488 and MitoView™633 in staurosporine-treated Jurkat cells. As apoptosis progresses, MitoView™633 staining is lost and cells become positive for NucView®488.

Mitochondrial Dyes

Abs/Em

Detection channel

Potential-dependent?

Catalog No.

Size

MitoView™ 633622/648 nm*Cy®5, APC*Yes70055-T50 ug
7005520×50 ug
NucView® 488 & MitoView™ 633 Apoptosis Kit500/530 nm (NucView®488)
622/648 nm* (MitoView­™ 633)
FITC/Cy®5*Yes30062100 assays
MitoView™ 405398/440 nmDAPIPartial†70070-T50 ug
7007020×50 ug
MitoView™ Green490/523 nmFITC, GFPNo70054-T50 ug
7005420×50 ug
JC-1 Mitochondrial Membrane Potential Detection Kit510/527 nm (cytoplasm)
585/590 nm (mitochondria)
FITC/Cy®3Yes30001100 assays
JC-1 Chloride Salt700115 mg
JC-1 Iodide Salt700145 mg
Rhodamine 123505/534 nmFITCYes7001050 mg
TMRE548/573 nmCy®3Yes7001625 mg
TMRE, 2 mM in DMSO700160.5 mL
TMRM548/573 nmCy®3Yes7001725 mg

*MitoView™ 633 also has red fluorescence in the Cy®3 channel and is not recommended for use with other red dyes.


Apoptosis/Necrosis Kits & Annexin V

Annexin V is a 36 kDa protein that has high affinity for phosphatidylserine (PS). During apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be stained by fluorescent conjugates of Annexin V. Annexin V conjugates are available with a wide selection of CF® dyes and other labels, including Near-IR CF® Dye Annexin V conjugates.

We offer many different staining kits with various combinations of dyes, including: NucView® caspase-3 substrate, our bright and photostable CF®488A Annexin V, a variety of dead cell nucleic acid stains, including propidium iodide (PI), 7-AAD, Ethidium Homodimer III (EthDIII), and RedDot™2. See the table below for more details on the various combination kits.

We offer dead cell nucleic acid stains separately as well, including far-red fluorescent RedDot™2 far-red dye and green fluorescent NucSpot® 470 stain.


Figure 12. Untreated and staurosporine-treated Jurkat cells stained with the Apoptotic, Necrotic and Healthy Cells Plus Kit. Apoptotic cells are stained green, necrotic/late apoptotic cells are stained red, and all cell nuclei are stained blue.

Apoptosis and Necrosis Staining Kits

Catalog No.

Features

CF®488A Annexin V and PI Apoptosis Kit30061• CF®488A stains apoptotic cells green for the FITC channel
• PI stains necrotic cells red for the Cy®3 or PE channels
• For microscopy or flow cytometry
CF®488A Annexin V and 7-AAD Apoptosis Kit30060• CF®488A stains apoptotic cells green for the FITC channel
• 7-AAD stains necrotic cells red for the PE-Cy®5 or FL3 channels
• For flow cytometry
Apoptosis & Necrosis Quantitation Kit Plus30065• CF®488A stains apoptotic cells green for the FITC channel
• EthD-III stains dead cells red for the Cy®3 or PE channels
• EthD-III has higher affinity and quantum yield than PI or 7-AAD
• For microscopy or flow cytometry
Apoptotic, Necrotic & Healthy Cells Quantitation Kit Plus30066• CF®488A stains apoptotic cells green for the FITC channel
• EthD-III stains dead cells red for the Cy® channel
• Hoechst stains all cell nuclei blue for the DAPI channel
• For microscopy
NucView®488 and RedDot™2 Apoptosis and Necrosis Kit30072• NucView®488 stains apoptotic nuclei green for the FITC channel
• RedDot™2 stains necrotic cells far-red for the Cy®5 or PE-Cy®5, APC, or FL3 channels
• For microscopy or flow cytometry
Annexin V ConjugatesMultiple• Annexin V with our bright and photostable CF® dyes and a selection of other labels
Annexin V Conjugates, Azide-FreeMultiple• Annexin V with our bright and photostable CF® dyes and a selection of other labels
• Lyophilized and preservative-free for real-time cell imaging
Annexin V Near-IR CF® Dye ConjugatesMultiple• Annexin V conjugated to our superior Near-IR CF® dyes
• Lyophilized and preservative-free for in vivo use
Annexin Binding Buffer99902• Concentrated Annexin Binding Buffer for cell staining
NucSpot® 470 Nuclear Stain40083• Green fluorescent dead cell stain
• Also useful as a nuclear-specific counterstain for fixed cells
RedDot™2 Far-Red Nuclear Stain40061• Far-red nuclear stain for dead cells or fixed cells
• Detect in the Cy®5 channel
Ethidium homodimer III40051• Red fluorescent dead cell stain
• Higher affinity and quantum yield than PI
Propidium Iodide40017• Widely used red fluorescent dead cell stain
7-AAD40037• Fluorescent dead cell stain for the PE-Cy®5/FL3 channel
NucSpot® Far-Red40085• Designed as an improved replacement for 7-AAD
• Less bleed into the PE-Texas Red® channel compared to 7-AAD

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BUFFERS, FIXATIVES, OTHER ACCESSORIES

We offer the following accessory products for your flow cytometry experiments:

Click here to see all of our flow cytometry accessory products.

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Live-or-Dye™ Fixable Viability Staining Kits

From: $65 Sizes: 50 assays, 200 assays, 250 assaysCatalog #: 32016, 32017, 32002-T, 32014-T, 32003-T, 32009-T, 32004-T, 32012-T, 32015-T, 32005-T, 32007-T, 32006-T, 32013-T, 32008-T, 32011-T, 32002, 32014, 32003, 32009, 32004, 32012, 32005, 32006, 32015, 32007, 32013, 32008, - 32011View allHide

ViaFluor® SE Cell Proliferation Kits

From: $50 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #: 30050, 30068, 30086, 30068-T, - 30086-TView allHide

NucView® Caspase-3 Enzyme Substrates

From: $67 Sizes: 10 uL (1 mM in DMSO), 100 uL (1 mM in DMSO), 10 uL (1 mM in PBS), 100 uL (1 mM in PBS)Catalog #: 10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide

Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

From: $362 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

MitoView™ Mitochondrial Dyes

From: $40 Sizes: Trial size (50 ug), 20 x 50 ugCatalog #: 70070-T, 70070, 70054-T, 70054, 70055-T, 70055, 70075-50ug, 70075, 70068-T, - 70068View allHide

Flow Cytometry Fixation/Permeabilization Kit

From: $28 Sizes: 50 testsCatalog #: 23006

NucSpot® Far-Red, 1000X in DMSO

Sizes: 50 uL, 0.5 mLCatalog #: 40085, - 40085-TView allHide